LOCUS       sequence-156607-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wagner E, Glotzer M
  TITLE     Local RhoA activation induces cytokinetic furrows independent of 
            spindle position and cell cycle stage.
  JOURNAL   J Cell Biol. 2016 Jun 20;213(6):641-9. doi: 10.1083/jcb.201603025. 
            Epub 2016 Jun 13.
  PUBMED    27298323
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201603025"; journalName: "J Cell Biol"; date: 
            "2016-06-20- 20"; volume: "213"; issue: "6"; pages: "641-9"
COMMENT     Sequence Label: PR_GEF  (2XPDZ-mCherry-Larg (DH)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    675..727
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      735..744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(886..904)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttatcg
       61 ataccgtcga catggcaaaa caagagattc gagtgagggt tgaaaaggac ccagaacttg
      121 gatttagcat atcaggtggt gtcgggggta gaggaaaccc attcagacct gatgatgatg
      181 gtatatttgt aacaagggta caacctgaag gaccagcatc aaaattactg cagccaggtg
      241 ataaaattat tcaggctaat ggctacagtt ttataaatat tgaacatgga caagcagtgt
      301 ccttgctaaa aactttccag aatacagttg aactcatcat tgtacgagaa gttagtgctg
      361 gtggtagtgc aggagggagt gctggcgggg caaaacaaga gattcgagtg agggttgaaa
      421 aggacccaga acttggattt agcatatcag gtggtgtcgg gggtagagga aacccattca
      481 gacctgatga tgatggtata tttgtaacaa gggtacaacc tgaaggacca gcatcaaaat
      541 tactgcagcc aggtgataaa attattcagg ctaatggcta cagttttata aatattgaac
      601 atggacaagc agtgtccttg ctaaaaactt tccagaatac agttgaactc atcattgtac
      661 gagaagtttc tagactcgag ctcaagcttc gaattctgca atcgacggta ccgcgggccc
      721 gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
      781 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      841 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      901 ccaagggtgg ccccctgccc
//
