LOCUS       sequence-156610-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Wagner E, Glotzer M
  TITLE     Local RhoA activation induces cytokinetic furrows independent of 
            spindle position and cell cycle stage.
  JOURNAL   J Cell Biol. 2016 Jun 20;213(6):641-9. doi: 10.1083/jcb.201603025. 
            Epub 2016 Jun 13.
  PUBMED    27298323
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201603025"; journalName: "J Cell Biol"; date: 
            "2016-06-20- 20"; volume: "213"; issue: "6"; pages: "641-9"
COMMENT     Sequence Label: PR_GEF YFP (2XPDZ-YFP-LARG DH)
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..353
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     290..311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      121 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      241 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagctcgagc
      361 tcaagcttag tgctggtggt agtgcaggag gctcggccgg tggaagtgca ggagggagtg
      421 ctggcggggt cgacgacacc ccacccaact ggcagcagct tgttagtcga gaagtgttac
      481 tgggactaaa accttgtgaa atcaaaagac aggaagtgat taatgaattg ttctacactg
      541 aaagagctca tgttcgaaca ctgaaggttc ttgatcaagt gttctatcag cgagtatcca
      601 gagaaggaat tctgtcaccc tcagagctac ggaaaatttt ttcaaacttg gaagatattc
      661 ttcaacttca tattggattg aatgaacaaa tgaaggctgt tcgaaagaga aatgagacct
      721 ctgttatcga tcagattggg gaagatttgc tgacatggtt cagcggacca ggagaggaga
      781 aattgaaaca tgctgctgct accttttgca gtaaccaacc tttcgcc
//
