LOCUS       sequence-156612-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector that expresses a VSV-G protein with a 
            FLAG tag near the N-terminus of the protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, 
            Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van 
            Calenbergh S, Gevaert K, Tavernier J
  TITLE     Trapping mammalian protein complexes in viral particles.
  JOURNAL   Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416.
  PUBMED    27122307
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-28"; volume: "7:11416. doi"; pages: " 
            10.1038/ncomms11416"
COMMENT     Sequence Label: pcDNA3-FLAG-VSVG
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(133..165)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagactc
      121 gagttagaat tcctttccaa gtcggttcat ctctatgtct gtataaatct gtcttttctt
      181 ggtgtgcttt aatttaatgc aaagatggat accaactcgg agaaccaaga atagtccaat
      241 gattaaccct atgataaaga aaaaagaggc aatagagctt ttccaactac tgaaccaacc
      301 ttctacaagc tcgattggat ttttggatag cccagtatca ccaaaaaata aactctcatc
      361 atcaggaagt tgcgaagcag cgtcttgaat gtgaggatgt tcgaacacct gagcctttga
      421 gctaagatga agatcggagt ccaacatacc atgtccaatc atgtataaag gaaacttata
      481 tcctgaactg gtcctcagaa ctccattggg tccaatttcc acgtcttcat atggtgccca
      541 gtcatcccac agttcccttt ctgtggtagt tccactgatc attccgacca ttcttgagag
      601 gattggagca gcaatatcga ctctgatgta tctggtctca aagtatttta gggtaccatt
      661 gattatggtg aaagcaggac cggttcctgg gtttttagga gcaagatagc tgagatccac
      721 tggagagatt ggaagacccg ctctgatttt gctccaggtt tcttggcaga gggaataatc
      781 caagatcctc tcaacgtcct gaattagact tacatccact gaggtctgag atggagcaga
      841 gatacttgac ccttctgggc attcagggaa tctggctgca gcaaagagat ccttatcagc
      901 catctcgaac cagacacctg atgggagtct gactccccaa tgcttgcagt attgcatttt
      961 gcaggccttg cctccagttt cataagcaaa gtagttactt ctgaaccctg tgccctcctt
     1021 tcccagggat ga
//
