LOCUS       sequence-156613-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector that expresses a VSV-G protein with a 
            FLAG tag near the N-terminus of the protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, 
            Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van 
            Calenbergh S, Gevaert K, Tavernier J
  TITLE     Trapping mammalian protein complexes in viral particles.
  JOURNAL   Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416.
  PUBMED    27122307
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-28"; volume: "7:11416. doi"; pages: " 
            10.1038/ncomms11416"
COMMENT     Sequence Label: pcDNA3-FLAG-VSVG
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             182..205
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagccc accatgaagt gccttttgta
      121 cttagccttt ttattcattg gggtgaattg caagttcacc atagtttttc catccggagg
      181 agattacaag gatgacgacg ataagggcgg aagcttggga cacaaccaaa aaggaaactg
      241 gaaaaatgtt ccttctaatt accattattg cccgtcaagc tcagatttaa attggcataa
      301 tgacttaata ggcacagcct tacaagtcaa aatgcccaag agtcacaagg ctattcaagc
      361 agacggttgg atgtgtcatg cttccaaatg ggtcactact tgtgatttcc gctggtatgg
      421 accgaagtat ataacacatt ccatccgatc cttcactcca tctgtagaac aatgcaagga
      481 aagcattgaa caaacgaaac aaggaacttg gctgaatcca ggcttccctc ctcaaagttg
      541 tggatatgca actgtgacgg atgccgaagc agtgattgtc caggtgactc ctcaccatgt
      601 gctggttgat gaatacacag gagaatgggt tgattcacag ttcatcaacg gaaaatgcag
      661 caattacata tgccccactg tccataactc tacaacctgg cattctgact ataaggtcaa
      721 agggctatgt gattctaacc tcatttccat ggacatcacc ttcttctcag aggacggaga
      781 gctatcatcc ctgggaaagg agggcacagg gttcagaagt aactactttg cttatgaaac
      841 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg
      901 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc
      961 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga
     1021 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg
     1081 tcttccaatc tctccagtgg atctcagcta tcttg
//
