LOCUS       sequence-156614-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression vector for DsRED monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kodama Y, Suetsugu N, Kong SG, Wada M
  TITLE     Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the 
            chloroplast photorelocation movement velocity in Arabidopsis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19591-6. doi: 
            10.1073/pnas.1007836107. Epub 2010 Oct 25.
  PUBMED    20974974
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1007836107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-11-9- 9"; volume: "107"; issue: "45"; pages: "19591-6"
COMMENT     Sequence Label: 35S-DsREDmonomer-NosT
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    33..88
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..804
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     525..544
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tttggagagg acaggcttct tgagatcctt caacaattac caacaacaac aaacaacaaa
       61 caacattaca attactattt acaattacag tcgactctag aggatccccg ggtaccggtc
      121 gccaccatgg acaacaccga ggacgtcatc aaggagttca tgcagttcaa ggtgcgcatg
      181 gagggctccg tgaacggcca ctacttcgag atcgagggcg agggcgaggg caagccctac
      241 gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc cttcgcctgg
      301 gacatcctgt ccccccagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac
      361 atccccgact acatgaagct gtccttcccc gagggcttca cctgggagcg ctccatgaac
      421 ttcgaggacg gcggcgtggt ggaggtgcag caggactcct ccctgcagga cggcaccttc
      481 atctacaagg tgaagttcaa gggcgtgaac ttccccgccg acggccccgt aatgcagaag
      541 aagactgccg gctgggagcc ctccaccgag aagctgtacc cccaggacgg cgtgctgaag
      601 ggcgagatct cccacgccct gaagctgaag gacggcggcc actacacctg cgacttcaag
      661 accgtgtaca aggccaagaa gcccgtgcag ctgcccggca accactacgt ggactccaag
      721 ctggacatca ccaaccacaa cgaggactac accgtggtgg agcagtacga gcacgccgag
      781 gcccgccact ccggctccca gtagagcggc cgcccggctg cagatcgttc aaacatttgg
      841 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
      901 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
      961 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     1021 tagcgcgcaa actaggataa attatcgcgc gcgg
//
