LOCUS       sequence-156616-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression vector for DsRED monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kodama Y, Suetsugu N, Kong SG, Wada M
  TITLE     Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the 
            chloroplast photorelocation movement velocity in Arabidopsis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19591-6. doi: 
            10.1073/pnas.1007836107. Epub 2010 Oct 25.
  PUBMED    20974974
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1007836107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-11-9- 9"; volume: "107"; issue: "45"; pages: "19591-6"
COMMENT     Sequence Label: 35S-DsREDmonomer-NosT
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..673)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GS"
     primer_bind     complement(256..275)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    712..767
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(789..835)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(824..844)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gggagccgga gtggcgggcc tcggcgtgct cgtactgctc caccacggtg tagtcctcgt
       61 tgtggttggt gatgtccagc ttggagtcca cgtagtggtt gccgggcagc tgcacgggct
      121 tcttggcctt gtacacggtc ttgaagtcgc aggtgtagtg gccgccgtcc ttcagcttca
      181 gggcgtggga gatctcgccc ttcagcacgc cgtcctgggg gtacagcttc tcggtggagg
      241 gctcccagcc ggcagtcttc ttctgcatta cggggccgtc ggcggggaag ttcacgccct
      301 tgaacttcac cttgtagatg aaggtgccgt cctgcaggga ggagtcctgc tgcacctcca
      361 ccacgccgcc gtcctcgaag ttcatggagc gctcccaggt gaagccctcg gggaaggaca
      421 gcttcatgta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtactgga
      481 actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc acctgcagct
      541 tggcggtctg ggtgccctcg tagggcttgc cctcgccctc gccctcgatc tcgaagtagt
      601 ggccgttcac ggagccctcc atgcgcacct tgaactgcat gaactccttg atgacgtcct
      661 cggtgttgtc catggtggcg accggtaccc ggggatcctc tagagtcgac tgtaattgta
      721 aatagtaatt gtaatgttgt ttgttgtttg ttgttgttgg taattgttga aggatctcaa
      781 gaagcctgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
      841 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
      901 tgaagacgtg gttggaacgt cttctttttc cacgatgttc ctcgtgggtg ggggtccatc
      961 tttggga
//
