LOCUS       sequence-156619-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Young TL, Cepko CL
  TITLE     A role for ligand-gated ion channels in rod photoreceptor 
            development.
  JOURNAL   Neuron. 2004 Mar 25. 41(6):867-79.
  PUBMED    15046720
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2004 Mar 25. 41(6):867-79."
COMMENT     Sequence Label: pGFP Glra2 KA (CC#303)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(155..157,158..160,161..874)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(155..157,158..160,161..871)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(200..221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(461..480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     808..829
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt
      181 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      241 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      301 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      361 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      421 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      481 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      541 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      601 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      661 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      721 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      781 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      841 gatcactctc ggcatggacg agctgtacaa gtaaagcgcc agcacagtgg tcgacgataa
      901 aa
//
