LOCUS       sequence-156635-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  create nuclear protein aggregates in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, 
            Wandless TJ
  TITLE     A method to rapidly create protein aggregates in living cells.
  JOURNAL   Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.
  PUBMED    27229621
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-05-27"; volume: "7:11689. doi"; pages: " 
            10.1038/ncomms11689"
COMMENT     Sequence Label: NLS-AgDD
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..729)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(19..729)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(59..79)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     674..702
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agtttagtta gttacttatt tgtagagctc atccatgcca tgtgtaatcc cagcagcagt
       61 tacaaactca agaaggacca tgtggtcacg cttttcgttg ggatctttcg aaaggacaga
      121 ttgtgtcgac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
      181 ttgttgataa tggtctgcta gttgaacgga accatcttca acgttgtggc gaattttgaa
      241 gttagctttg attccattct tttgtttgtc tgccgtgatg tatacattgt gtgagttaaa
      301 gttgtactcg agtttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccctttaa
      361 ctcgatacga ttaacaaggg tatcaccttc aaacttgact tcagcacgcg tcttgtaggt
      421 cccgtcatct ttgaaagata tagtgcgttc ctgtacataa ccttcgggca tggcactctt
      481 gaaaaagtca tgccgtttca tgtgatccgg ataacgggaa aagcattgaa caccataggt
      541 cagagtagtg acaagtgttg gccacggaac aggtagtttt ccagtagtgc aaataaattt
      601 aagggtgagt tttccgtttg tagcatcacc ttcaccctct ccacggacag aaaatttgtg
      661 cccattaaca tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc
      721 tcctttgctg aattcttc
//
