LOCUS       sequence-156636-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  create nuclear protein aggregates in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, 
            Wandless TJ
  TITLE     A method to rapidly create protein aggregates in living cells.
  JOURNAL   Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.
  PUBMED    27229621
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-05-27"; volume: "7:11689. doi"; pages: " 
            10.1038/ncomms11689"
COMMENT     Sequence Label: NLS-AgDD
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..369
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     primer_bind     complement(403..431)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagaagaaac gaaaagtact ggccctgaag ctggctggtc tagatattgg agtgcaggtg
       61 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgtgtggtg
      121 cactacaccg ggatgcttga agatggaaag aaagtcgatt cctcccggga cagaaacaag
      181 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
      241 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
      301 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
      361 aaaccggaag aattcagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      421 gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat
      481 gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg
      541 tggccaacac ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat
      601 cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc
      661 actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt
      721 gatacccttg ttaatcgtat cgagttaa
//
