LOCUS       sequence-156644-        5628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pBAD-TDsmURFP-RBS-HO-1
FEATURES             Location/Qualifiers
     source          1..5628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             312..334
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1211..1228
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2028..2045)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2198..2284
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2376..2403
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2423..2514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2515..2583,2584..3375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2733..2752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3546..4134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4035..4054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4288..4305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4320..4460
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4386..4405)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4546..4568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4724..5602)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctgt
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagccg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tcgatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatatgga tccatgaaaa cttctgaaca
      361 acgtgtaaac atcgcaactc tgctgactga gaacaaaaag aaaatcgttg ataaggcgtc
      421 tcaggatctg tggcgtcgtc atccagatct cattgcgccg ggcggcatcg cttttagtca
      481 acgcgatcgt gcactgtgcc tgcgtgacta cggctggttt ctgcacctga tcacgttctg
      541 tctgctggct ggtgataaag gtcctattga atccatcggt ctaattagca tacgcgaaat
      601 gtacaacagc ctgggtgtgc cggttccggc tatgatggaa tctatccgtt gtctgaaaga
      661 ggcctccctg tcactgctgg acgaggaaga cgccaatgag actgcaccgt actttgacta
      721 tatcatcaag gctatgtccg ggcatggcac cggcagcacc ggcagcggca gctccggcac
      781 cgcctcctcc gaggacaaca acatggccat gaaaacttct gaacaacgtg taaacatcgc
      841 aactctgctg actgagaaca aaaagaaaat cgttgataag gcgtctcagg atctgtggcg
      901 tcgtcatcca gatctcattg cgccgggcgg catcgctttt agtcaacgcg atcgtgcact
      961 gtgcctgcgt gactacggct ggtttctgca cctgatcacg ttctgtctgc tggctggtga
     1021 taaaggtcct attgaatcca tcggtctaat tagcatacgc gaaatgtaca acagcctggg
     1081 tgtgccggtt ccggctatga tggaatctat ccgttgtctg aaagaggcct ccctgtcact
     1141 gctggacgag gaagacgcca atgagactgc accgtacttt gactatatca tcaaggctat
     1201 gtccgaattc catcatcacc atcaccattg aaagaaggag atatacatac ccatgagtgt
     1261 caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg cggagaacgt
     1321 cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc gtaagctggt
     1381 tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat ttaaggacca
     1441 tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc tagagcaaga
     1501 cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg ccgctggcca
     1561 agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt tggtggccca
     1621 ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga aaattgccca
     1681 aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg acattgatga
     1741 cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca ttgaccaagc
     1801 caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca tgaaaatgtt
     1861 caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca acagcctcac
     1921 ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag gctaggttta
     1981 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
     2041 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     2101 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     2161 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     2221 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     2281 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     2341 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     2401 ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg
     2461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggtagagcgc
     4321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgtat atggtgcact
     4381 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     4441 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
     4621 aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     4681 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     4741 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     4801 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     4861 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     4921 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     4981 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     5041 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     5101 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     5161 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     5221 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     5281 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     5341 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     5401 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     5461 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     5521 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     5581 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagag
//
