LOCUS       sequence-156649-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..170)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(198..596)
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
     primer_bind     complement(621..640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(622..640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(685..888)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(714..734)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat cgtacgaggg
      121 ccgggattct cctccacgtc accgcatgtt agaagacttc ctctgccctc accgccacca
      181 gatccacctc cgctagcgct catagcctta ataatgtaat caaagtaggg tgcggtctcg
      241 ttggcgtcct cttcgtccag cagggacagg ctggcttctt tcaggcatct aatgctctcc
      301 atcattgcag ggacgggcac tcccagtgag ttatacattt ctcgaatact gatcagccca
      361 atagactcga tggggccctt atctccggcc agcagacaaa aggtgatcag atgcaggaac
      421 cagccgtaat ctcgcaggca cagagcgcgg tccctctgag agaaagcaat tcctcctggt
      481 gcgatcaggt ctgggtgtct ccgccacaga tcctgggaag ccttatccac gattttcttc
      541 ttattttctg tcagcagtgt agcaatgttc accctctgtt cggaagtctt agccatggtg
      601 tcggatccca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      661 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      721 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      781 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      841 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      901 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
      961 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggca
//
