LOCUS       sequence-156650-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..759)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(327..346)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..614
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(766..819)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt acttgtacag
       61 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      121 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
      181 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
      241 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
      301 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      361 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
      421 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      481 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
      541 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
      601 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
      661 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
      721 gatgatggcc atgttatcct cctcgccctt gctcaccatc gtacgagggc cgggattctc
      781 ctccacgtca ccgcatgtta gaagacttcc tctgccctca ccgccaccag atccacctcc
      841 gctagcgctc atagccttaa taatgtaatc aaagtagggt gcggtctcgt tggcgtcctc
      901 ttcgtccagc agggacaggc tggcttcttt caggcatcta atgctctcca tcattgcagg
      961 ga
//
