LOCUS       sequence-156655-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP and mCardinal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP-T2A-mCardinal
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..783)
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             complement(790..843)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt acttgtacag
       61 ctcgtccatg ccattaagtt tgtgccccag tttgctaggg aggtcgcagt atctggccac
      121 agccacctcg tgctgctcga cgtaggtctc attgtcggcc tccttgattc tttccagtct
      181 gcggtccaca aagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta
      241 tgtggtcttc aggttgcagt gcaggtggcc cccgcccacg agcttcaggg ccatgtcgca
      301 tctgccttcc aggccgccgt cagcggggta cagggtctcg gtggtggcct cccagccgag
      361 tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga gcttgacgtt
      421 gtagatcaag cagccgtcct ggaggctggt gtcctgggta acggtaagca cgcccccgtc
      481 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaaatc
      541 ggggatgccc tgggtgtggt tgatgaaggt cttgctcccg tacataaagc aggtggccag
      601 gatgtcgaat gcgaacggca gggggcctcc ctccaccacc ttaatcctct gggtctgggt
      661 gccctcgtag ggcttgccct ccccttcggt ggtgcacttg aagtggtggt tgttcacggt
      721 gccttccatg tacagcttca tgtgcatgtt ctccttgatc agctcctcgc ccttgctcac
      781 catcgtacga gggccgggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc
      841 ctcaccgcca ccagatccac ctccgctagc gctcatagcc ttaataatgt aatcaaagta
      901 gggtgcggtc tcgttggcgt cctcttcgtc cagcagggac aggctggctt ctttcaggca
      961 tctaatgctc tccatcattg cagggacggg cactcccagt gagttataca tttctcgaat
     1021 actgatcagc ccaatagact cgatggg
//
