LOCUS       sequence-156665-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a targeting vector to target the endogenous Sox2 SE CPG island with 
            Snrp-Tomato-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.08"; journalName: "Cell"; date: "2015-09-24- 
            24"; volume: "163"; issue: "1"; pages: "218-29"
COMMENT     Sequence Label: SOX2se-CPG-TV
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             926..2356
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2422..3010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2475..2495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2893..2904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3013..3029)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3014..3030)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3025..3042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3031..3238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3291..3324
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3385..3881
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3420..3443)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3814..3833
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3921..4520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3921..3940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4417..4437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5077..5110
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5979..5998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5980..5998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6005..6022)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6005..6021)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6014..6036)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6162..6590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6249..6268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6459..6480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6934..7728
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6988..7007)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7598..7617
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7746..7814,7815..8606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7964..7983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8777..9365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9266..9285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9519..9536
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg taccgagctc
       61 ggatccacta gtaacggccg ccagtgtgct ggaattcgcc cttttcctgc aggccggggt
      121 ttcctgatct cttgcccaag tggctgctgt gcccagcatg tgggcccaat ttattttcct
      181 actttttcca ggatgcgcag atgcaggaac tatctttggt gtttctgtct ttctgcctgc
      241 tcaccgcagt ttccgctaac aaaacaagcc tttgtgcact ttgtttgcag tgttgagaac
      301 tgatcacagg gacgtgggag aacccctccc tggagccgcg cgcggcgacc ctttctgtgg
      361 atctggctta ctgaggctca gtccgctttg ccacaccgcc tcctgctggc ggctagttga
      421 cctgccgcgc tttttcttct gtgcgtcaga tacttcacca ggcctgaggg tcttctcttt
      481 tcttttgaaa tagggctcaa ggaacccagg cagactgcaa actcactaag tggcccgaag
      541 ctgactttga actcatgatc ttcttgcatc cattttcgtg gagctggggt tgcccatgca
      601 taccaccaca cccagctttc cgagccagac aattgacgct caaatttccg cagtaggaat
      661 gctcaagcat tccttttggt agctgccttt tggcaggaca ttccggtcag agggacagag
      721 acccctgcat tgcggcaaaa atgtgcgcat gtgcagccat tgcctgggac gcatgcgtag
      781 ggagccgcgc gacaaacctg agccattgcg gcaagactag cgcagagagg agagggagcc
      841 ggagatgcca gacgcttggt tctgaggagt gatttgcaac gcaatggagc gaggaaggtc
      901 agctgggctt gtggattctg ctagcatggt gagcaagggc gaggaggtca tcaaagagtt
      961 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     1021 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1081 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     1141 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     1201 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     1261 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     1321 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     1381 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     1441 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     1501 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     1561 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     1621 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     1681 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1801 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1861 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1981 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     2041 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     2101 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     2161 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     2221 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     2281 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     2341 cgagctgtac aagtaagaat tgtgttgcac ttaacgcgta caaggccggc cctgcaggaa
     2401 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     2461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2821 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2941 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3001 gcctccccgc atcgataccg tcgacctcga ctgtgccttc tagttgccag ccatctgttg
     3061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggaac
     3241 tagtggtgcc agggcgtgtt aattaacgat catattcaat aacccttaat ataacttcgt
     3301 ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagctta
     3361 ggatctcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     3421 catgcgcttt agcagccccg ctggcacttg gcgctacaca agtggcctct ggcctcgcac
     3481 acattccaca tccaccggta gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     3541 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     3601 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     3661 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     3721 tgggctcagc agctgggaag ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3781 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3841 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc
     3901 tcgagcagct gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3961 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     4021 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     4081 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     4141 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     4201 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     4261 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     4321 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     4381 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     4441 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4501 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     4561 cacgacccca tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa
     4621 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
     4681 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
     4741 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
     4801 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
     4861 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
     4921 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
     4981 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
     5041 tccatcagaa gctggtcgag atccggaacc cttaatataa cttcgtataa tgtatgctat
     5101 acgaagttat taggtccctc gaagaggttc actaggcgcg ccggaggggg ctgcattctc
     5161 agacaactct cagtggctag cttgcccttt gacaaaactt tgctaggaat ttaccacccc
     5221 taccccccct ccccagactc tccctttgta gctcctctct tcccttctct ctcacttggc
     5281 tccctgctca tttgactgac agcttgggca aagactcctc tcctgctgag ctgagaccct
     5341 gaagagaatt ccactaacac gtaaacaagg tcttggctga ccttgaactc actatgtaga
     5401 ccctgcaggt ctcctattct gacttctaaa ttccgggatt aaaggcatca ccacgcctag
     5461 tcattgatat gtcttattat ttattttatg agtatgaata ttttgcctgt ttgtatgtct
     5521 gtgcaccatg tgcatgcctg gtgctaatgg aggccagagg agggcatcag gccctctgga
     5581 gctagagtta cagatggttg tgagcctcta tgcacatgct gggaccagaa cccaggtcct
     5641 ctgcaagagc agccagtgct cttaaccact gagccatctt ttcaactcct caatttccat
     5701 cttaaatttt attttgtgct gggctagcct tggctgtata ataagaccca gtttcaataa
     5761 gcaataaggt tcattttatg tgtgtcttag aggcttgtat atcacactgc ttaatctttt
     5821 attttggtgg ggtacaggac attgaaattg agagagggtt tcttgtaact gcagctggtc
     5881 tcgaacctgt ttcgtagcgg ccggccaata agggcgaatt ctgcagatat ccatcacact
     5941 ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag tcgtattaca
     6001 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6061 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     6181 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6241 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6301 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6361 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6421 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6481 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6541 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6601 tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg
     6661 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     6721 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     6781 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     6841 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
     6901 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6961 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7021 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7081 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7141 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7201 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca ccttgctcct
     7261 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7321 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7381 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7441 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7501 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7561 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7621 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7681 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga
     7741 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7801 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7861 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7921 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7981 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8041 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8101 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8161 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8221 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8281 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8341 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8401 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8461 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8521 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8581 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8641 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8701 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8761 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8821 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8881 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8941 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9001 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9061 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9421 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9481 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9541 cggaag
//
