LOCUS       sequence-156674-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-MDGA2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-MDGA2
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4144..4185
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7028..7616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7081..7101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7499..7510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7619..7635)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7620..7636)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8141..8321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8347..8364)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8353..8577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8623..9051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8710..8729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8920..8941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9060..9080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9065..9394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9245..9380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9307..9326
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9442..9489
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9508..9882
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10012..10133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10049..10068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10103..10122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10182..10198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10182..10198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10195..10217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10206..10222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10230..10236,10237..10254,10255..10260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10275..10296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10413..10430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10584..11172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10664..10683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11343..12134,12135..12203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11966..11985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12204..12308)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagac gccaccatgg atttcctgct gctcggtctc
     3121 tgtctatact ggctgctgag gaggccctcg ggggtggtct tgtgtctgct gggggcctgc
     3181 tttcagatgc tgcccgccgc tagccagctt actccaactt tctacgataa ctcttgtcct
     3241 aacgtgagca acatcgtgcg agatactatc gtgaacgagt tgcggtctga tccgaggata
     3301 gccgcttcaa ttttgcggct gcatttccac gattgttttg taaacggctg tgatgcttcc
     3361 atcctcctcg acaataccac ctctttcagg actgaaaagg atgccttcgg taacgcgaat
     3421 agtgctagag ggtttcccgt gatcgataga atgaaagccg ctgtggagag cgcctgcccc
     3481 cggacagtta gttgtgcaga ccttctgacc atagcggccc agcaatccgt gacgctggcc
     3541 ggtggcccca gctggagggt ccccctggga aggagggact ctctgcaggc ttttctggac
     3601 ttggcaaacg caaacctgcc tgctcccttc ttcacccttc cccagctgaa ggattccttc
     3661 aggaatgtcg gcctgaaccg aagtagtgat ttggtggctc tgtccggagg ccacacattc
     3721 ggcaagaatc agtgcaggtt catcatggac agactgtata acttttccaa caccggcttg
     3781 cctgacccta ccctgaacac aacatacttg cagactctca gagggctgtg tccgctcaat
     3841 ggcaacttgt ctgcactggt tgactttgac ctgcgcaccc caaccatctt cgacaataag
     3901 tattacgtca atctggagga acagaagggc ctgattcagt ccgaccagga gctgttcagt
     3961 tccccaaatg ctaccgacac tattcctctt gtgcgcagct tcgctaatag cacacaaacc
     4021 tttttcaatg ctttcgtgga agctatggat cggatgggaa atataacgcc cctgacgggt
     4081 acgcagggac agataagact gaactgtagg gtcgtgaaca gcaactctgg ctcgggctcg
     4141 ggcggcaagc ccatccccaa ccccctgctg ggcctggaca gcaccaccgg tcccccgacg
     4201 gttcggattg tgcactcagg attggcctgt aatatagagg aggagcgcta ctcggaaagg
     4261 gtctatacca tccgggaagg agaaacgcta gagttgacct gtctggttac tggacatcca
     4321 cgcccacaga tcaggtggac caaaacagca ggaagcgcct ctgacagatt ccaagactca
     4381 agtgtcttca atgagacttt gaggattaca agcattcaga gacaccaagg aggccgatat
     4441 tactgtaaag cagagaatgg cttggggtct ccagcaataa agtcgatccg agtggatgtg
     4501 tactacttgg atgatcctgt agtaactgtt catcaaagta taggtgaagc taaagaacaa
     4561 ttttactatg agagaacagt gttcctccga tgtgtggcca attccaaccc ccctgtgcgc
     4621 tacagctgga gacgtggcca agaggtgttg ctacaaggat cagacaaagg agtggagatt
     4681 tacgagccct tttttaccca gggggaaaca aagatcttaa agctaaagaa tctccgacct
     4741 caggactatg ctaactacag ctgcattgct tcagtgagga acgtgtgtaa tattccagac
     4801 aagatggtgt cttttagact ttccaataaa accgcttctc cgtcaattaa gctcttggtg
     4861 gatgacccta tagttgtaaa tcctggagag gccataacgt tagtgtgtgt tacaacaggg
     4921 ggagagccta tgccctctct cacctgggtc aggtccttcg ggactctgcc tgaaaagatt
     4981 gttttgaatg gagggacatt gaccatacct gccatcacct ctgatgatgc tgggacttac
     5041 agctgcattg ccaataataa tgtgggaaac cctgcaaaaa agtccaccaa catcattgtg
     5101 agagcattaa aaaaaggacg attttggatc actcctgatc cttatcacaa agatgacaac
     5161 atccagattg ggcgtgaggt gaaaatatct tgccaagtag aagccgttcc ctctgaagag
     5221 ttaacattca gttggtttaa aaacggccgt ccattaagaa gttctgagcg gatggttatt
     5281 acacagactg accctgatgt gtcccccgga acaacaaatt tggatatcat tgatttaaaa
     5341 ttcacggact ttgggactta cacatgcgtg gcctctctga agggaggagg aatatctgat
     5401 atcagtattg acgttaacat ttccagcagc acagttccac ccaatctgac tgttccacag
     5461 gaaaaatcac ctttggtcac cagagaaggg gacacaatag aacttcagtg tcaagtaact
     5521 gggaaaccaa aaccaatcat cctttggtct agagcagata aagaagttgc aatgcctgat
     5581 gggacaatgc aaatggagag ttatgatggg accctcagga ttgtgaacgt gtcaagggaa
     5641 atgtctggga tgtacagatg ccagaccagt cagtacaatg gattcaacgt gaagcccaga
     5701 gaagccttgg tgcagctcat cgtgcagtat cccccagctg tggaaccagc attcttggaa
     5761 atccgtcaag gacaagacag aagtgtgacc atgagctgta gggttctaag agcctatccg
     5821 atacgtgtgc tgacttacga atggcgtctg ggcaataaat tattacggac cggtcagttt
     5881 gactctcagg agtatactga gtacccacta aagagtcttt ccaatgaaaa ctatggagtt
     5941 tataactgca gcatcattaa tgaagctgga gcagggagat gcagctttct tgtgacagga
     6001 aaagcctatg ccccagagtt ctactatgac acctacaacc ctgtgtggca gaaccgacac
     6061 cgagtgtatt cttacagtct gcagtggaca caaatgaatc ccgatgcagt ggatcggatt
     6121 gtcgcatacc gtttgggtat tcggcaggct ggacagcaac gttggtggga acaggagatt
     6181 aaaataaatg ggaatattca aaagggagaa ctaattacat ataacttgac tgaacttata
     6241 aaaccagagg cctatgaagt ccgactgact cctctcacta aatttggtga aggagattca
     6301 acaattcgtg taattaaata tacggcacct gtaaatcctc atttgagaga atttcactgt
     6361 ggatttgaag atggtaatat atgcttattc acccaagatg atacagataa ctttgactgg
     6421 acaaagcaaa gtactgcaac aagaaataca aaatacaccc ctaacacagg tcccaatgca
     6481 gatcggagtg gttccaaaga aggtttttat atgtacatcg agacatcgcg acccagacta
     6541 gaaggagaaa aggctcgact tctcagccct gtattcagca tagctcccaa aaatccatat
     6601 ggacctacca atagtgcata ttgtttcagt ttcttctatc acatgtatgg gcaacatata
     6661 ggtgttttaa atgtatatct acgtttgaaa gggcagacga caatagagaa tcctctatgg
     6721 tcttcgagtg ggaacaaagg acaacgatgg aatgaagctc atgttaatat atatccaatt
     6781 acttcatttc agttaatttt tgaaggcatt cggggtcctg ggatagaagg tgacatcgcc
     6841 attgacgatg tgtcaattgc agaaggagaa tgtgcaaaac aagacctacc aactaagaat
     6901 tccgtggatg gtgctgttgg gatcttagtt catatatggc tttttccagt tatcatcctc
     6961 atctcgatct taagccctcg aaggtgaggc gcgcccctgc agggaattcg atatcaagct
     7021 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7561 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7621 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7681 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7741 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7861 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     7921 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     7981 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     8041 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     8101 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     8341 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     8401 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     8461 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     8521 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     8581 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     8641 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8701 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8761 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8821 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8881 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8941 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9001 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     9061 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9301 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     9361 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     9421 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     9481 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     9541 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     9601 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     9661 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     9721 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     9781 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     9841 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     9901 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9961 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    10021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10141 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12301 gttccgcgca catttccccg aaaagtgcca cctgac
//
