LOCUS       sequence-156703-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of hM4Di-mCherry under CBA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Hou XH, Hyun M, Taranda J, Huang KW, Todd E, Feng D, Atwater E, 
            Croney D, Zeidel ML, Osten P, Sabatini BL
  TITLE     Central Control Circuit for Context-Dependent Micturition.
  JOURNAL   Cell. 2016 Sep 22;167(1):73-86.e12. doi: 10.1016/j.cell.2016.08.073.
  PUBMED    27662084
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "73-86.e12"
COMMENT     Sequence Label: pAAV-CBA-DIO-hM4Di-mCherry
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cgccaccatg
      181 gccaacttca cacctgtcaa tggcagctcg ggcaatcagt ccgtgcgcct ggtcacgtca
      241 tcatcccaca atcgctatga gacggtggaa atggtcttca ttgccacagt gacaggctcc
      301 ctgagcctgg tgactgtcgt gggcaacatc ctggtgatgc tgtccatcaa ggtcaacagg
      361 cagctgcaga cagtcaacaa ctacttcctc ttcagcctgg cgtgtgctga tctcatcata
      421 ggcgccttct ccatgaacct ctacaccgtg tacatcatca agggctactg gcccctgggc
      481 gccgtggtct gcgacctgtg gctggccctg gactgcgtgg tgagcaacgc ctccgtcatg
      541 aaccttctca tcatcagctt tgaccgctac ttctgcgtca ccaagcctct cacctaccct
      601 gcccggcgca ccaccaagat ggcaggcctc atgattgctg ctgcctgggt actgtccttc
      661 gtgctctggg cgcctgccat cttgttctgg cagtttgtgg tgggtaagcg gacggtgccc
      721 gacaaccagt gcttcatcca gttcctgtcc aacccagcag tgacctttgg cacagccatt
      781 gctggcttct acctgcctgt ggtcatcatg acggtgctgt acatccacat ctcc
//
