LOCUS       sequence-156719-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Accessory plasmid (encodes M13 gene VI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-PRM-B0034-geneVI
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     225..245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        227..245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(297..635)
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     RBS             642..653
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(765..781)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(766..782)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(806..825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(807..825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(835..852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(835..851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(844..866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      241 aaagggaaca aaagctggag ctgcgaaaaa accccgccga agcggggttt tttgcgttat
      301 ttatcccaat ccaaataaga aacgattttt tgtttaacgt caaaaatgaa aatagcagcc
      361 tttacagaga gaataacata aaaacaggga agcgcattag acgggagaat taactgaaca
      421 ccctgaacaa agtcagaggg taattgagcg ctaatatcag agagataacc cacaagaatt
      481 gagttaagcc caataataag agcaagaaac aatgaaatag caatagctat cttaccgaag
      541 ccctttttaa gaaaagtaag cagatagccg aacaaagtta ccagaaggaa accgaggaaa
      601 cgcaataata acggaatacc caaaagaact ggcatctagt atttctcctc tttacaagat
      661 acattgtaaa tctatacaag aaagtttgta atatctaaca ccgtgcgtgt tgactatttt
      721 acctctggcg gtgataatgg ttgcgaattc gatatcaagc ttatcgatac cgtcgacctc
      781 gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
      841 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      901 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      961 ccaacagttg cgcagcctga aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1021 tgtttatttt tctaa
//
