LOCUS       sequence-156720-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Accessory plasmid (encodes M13 gene VI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-PRM-B0034-geneVI
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..42
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     27..43
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             155..166
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             173..511
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     primer_bind     complement(563..583)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(602..618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(602..618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(615..637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    626..642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(650..656,657..674,675..680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    695..716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(833..850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
       61 ttcgcaacca ttatcaccgc cagaggtaaa atagtcaaca cgcacggtgt tagatattac
      121 aaactttctt gtatagattt acaatgtatc ttgtaaagag gagaaatact agatgccagt
      181 tcttttgggt attccgttat tattgcgttt cctcggtttc cttctggtaa ctttgttcgg
      241 ctatctgctt acttttctta aaaagggctt cggtaagata gctattgcta tttcattgtt
      301 tcttgctctt attattgggc ttaactcaat tcttgtgggt tatctctctg atattagcgc
      361 tcaattaccc tctgactttg ttcagggtgt tcagttaatt ctcccgtcta atgcgcttcc
      421 ctgtttttat gttattctct ctgtaaaggc tgctattttc atttttgacg ttaaacaaaa
      481 aatcgtttct tatttggatt gggataaata acgcaaaaaa ccccgcttcg gcggggtttt
      541 ttcgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      901 taatacggtt atcca
//
