LOCUS       sequence-156722-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-PR/PRM-GFP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     33..49
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(84..170)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(464..483)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     733..751
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat
       61 atcgaattcg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      121 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctctac
      181 tcgagttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
      241 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg
      301 ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg
      361 tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg
      421 tcctcggggt acatccgctc ggaggaggcc tcccagccca tagtcttctt ctgcattacg
      481 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
      541 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      601 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
      661 ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
      721 agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
      781 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
      841 aagcgcatga actccttgat gatagc
//
