LOCUS       sequence-156723-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-PR/PRM-GFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             712..723
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             817..828
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttcgtccatg ccgtgggtga tacccgcagc cgtcacaaat tccagcagga ccatgtggtc
       61 acgtttttcg ttcggatctt ttgacagcac ggactgggta ctcaggtagt gattgtccgg
      121 cagcaggacc gggccatcac caatcggcgt gttttgctga tagtggtcgg ccagctgaac
      181 gctaccatct tccacattat ggcggatttt gaaattggct ttaatgccgt ttttctgttt
      241 atccgcggtg atgtacacgt tgtgggaatt gaagttatat tccagtttat gacccagaat
      301 attgccgtct tctttgaaat cgataccttt cagttcgata cggttaacca gggtatcgcc
      361 ttcgaatttc acttccgcgc gggttttata cgtaccatcg tctttaaagc taatcgtacg
      421 ttcctgcaca tagccttccg gcatggccga tttgaaaaag tcgtgctgtt tcatgtgatc
      481 cgggtaacgc gcaaaacatt gaacgccata ggtcagcgtg gtcaccagcg tcggccacgg
      541 gaccggcagt ttacccgtgg tgcagataaa tttcagggtc agtttgccgt tcgtcgcatc
      601 gccttcacct tcgccgcgaa cactgaattt atgaccatta acatcgccgt ccagttccac
      661 cagaatcgga accacaccgg taaacagttc ttcgccttta cgcatctagt atttctcctc
      721 tttaatctct agtaacgtta aatctatcac cactatttat aaatatctaa caccgtgcgt
      781 gttgactatt ttacctctgg cggtgataat ggttgcaaag aggagaaaga attcatggtg
      841 agcaagggcg aggaggataa catggctatc atcaaggagt tcatgcgctt caaggtgcac
      901 atggagggct ccgtgaacgg ccacgagttc
//
