LOCUS       sequence-156726-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-P/PM,5C6A-GFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..53
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     38..54
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(89..175)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(469..488)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     738..756
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc
       61 ttgatatcga attcgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
      121 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      181 tctactcgag ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      241 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
      301 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
      361 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
      421 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatagtc ttcttctgca
      481 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
      541 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
      601 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
      661 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
      721 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      781 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
      841 ccttgaagcg catgaactcc ttgatgatag ccatgtt
//
