LOCUS       sequence-156728-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-P/PM,5G6G-GFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..54
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     39..55
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(90..176)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(470..489)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     739..757
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
       61 cttgatatcg aattcgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
      121 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
      181 ctctactcga gttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
      241 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      301 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
      361 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
      421 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatagt cttcttctgc
      481 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
      541 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
      601 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
      661 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
      721 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      781 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
      841 accttgaagc gcatgaactc cttgatgata gccatgt
//
