LOCUS       sequence-156729-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-P/PM,5G6G-GFP
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..720)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             727..738
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             821..832
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(984..1002)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcgttattta tacagttcgt ccatgccgtg ggtgataccc gcagccgtca caaattccag
       61 caggaccatg tggtcacgtt tttcgttcgg atcttttgac agcacggact gggtactcag
      121 gtagtgattg tccggcagca ggaccgggcc atcaccaatc ggcgtgtttt gctgatagtg
      181 gtcggccagc tgaacgctac catcttccac attatggcgg attttgaaat tggctttaat
      241 gccgtttttc tgtttatccg cggtgatgta cacgttgtgg gaattgaagt tatattccag
      301 tttatgaccc agaatattgc cgtcttcttt gaaatcgata cctttcagtt cgatacggtt
      361 aaccagggta tcgccttcga atttcacttc cgcgcgggtt ttatacgtac catcgtcttt
      421 aaagctaatc gtacgttcct gcacatagcc ttccggcatg gccgatttga aaaagtcgtg
      481 ctgtttcatg tgatccgggt aacgcgcaaa acattgaacg ccataggtca gcgtggtcac
      541 cagcgtcggc cacgggaccg gcagtttacc cgtggtgcag ataaatttca gggtcagttt
      601 gccgttcgtc gcatcgcctt caccttcgcc gcgaacactg aatttatgac cattaacatc
      661 gccgtccagt tccaccagaa tcggaaccac accggtaaac agttcttcgc ctttacgcat
      721 ctagtatttc tcctctttac gttaaatcta tcaccactat ttataaatat ctatcggcgc
      781 cgccgttgac tattttatcg gcggcgccga taatggttgc aaagaggaga aagaattcat
      841 ggtgagcaag ggcgaggagg ataacatggc tatcatcaag gagttcatgc gcttcaaggt
      901 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      961 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1021 cgcctgggac a
//
