LOCUS       sequence-156738-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid (mCherry and GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Brodel AK, Jaramillo A, Isalan M
  TITLE     Engineering orthogonal dual transcription factors for multi-input 
            synthetic promoters.
  JOURNAL   Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
  PUBMED    27982027
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-16"; volume: "7:13858. doi"; pages: " 
            10.1038/ncomms13858"
COMMENT     Sequence Label: pJPC12-Delta-M13-mCherry-P/PM,4A5C6G7G-GFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     36..52
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(87..173)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(189..899)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(467..486)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     736..754
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             906..917
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
       61 gatatcgaat tcgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
      121 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc
      181 tactcgagtt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      241 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      301 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      361 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      421 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatagtctt cttctgcatt
      481 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      541 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      601 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      661 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
      721 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      781 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
      841 ttgaagcgca tgaactcctt gatgatagcc atgttatcct cctcgccctt gctcaccatg
      901 aattctttct cctctttgca accattatac gggcccc
//
