LOCUS       sequence-156755-        8919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-ERBB2 A775_G776insYVMA
FEATURES             Location/Qualifiers
     source          1..8919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1926..2395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2460..2659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2860..3276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3206..3228
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3244..3260
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    3304..3328
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7106..7130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7167..7187)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7172..7501
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7352..7487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7511..8110
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7511..7530)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8007..8027
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8331..8800
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac
     3301 atcacaagtt tgtacaaaaa agttggcatg gagctggcgg ccttgtgccg ctgggggctc
     3361 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
     3421 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
     3481 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
     3541 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
     3601 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
     3661 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
     3721 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
     3781 ggggtcttga tccagcggaa cccccagctc tgctaccaag acacgatttt gtggaaggac
     3841 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
     3901 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
     3961 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
     4021 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
     4081 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
     4141 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
     4201 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
     4261 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
     4321 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
     4381 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
     4441 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
     4501 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
     4561 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
     4621 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
     4681 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
     4741 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
     4801 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
     4861 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
     4921 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
     4981 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
     5041 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
     5101 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
     5161 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
     5221 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
     5281 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
     5341 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
     5401 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
     5461 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
     5521 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
     5581 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca
     5641 tacgtgatgg catatgtcat ggctggtgtg ggctccccat atgtctcccg ccttctgggc
     5701 atctgcctga catccacggt gcagctggtg acacagctta tgccctatgg ctgcctctta
     5761 gaccatgtcc gggaaaaccg cggacgcctg ggctcccagg acctgctgaa ctggtgtatg
     5821 cagattgcca aggggatgag ctacctggag gatgtgcggc tcgtacacag ggacttggcc
     5881 gctcggaacg tgctggtcaa gagtcccaac catgtcaaaa ttacagactt cgggctggct
     5941 cggctgctgg acattgacga gacagagtac catgcagatg ggggcaaggt gcccatcaag
     6001 tggatggcgc tggagtccat tctccgccgg cggttcaccc accagagtga tgtgtggagt
     6061 tatggtgtga ctgtgtggga gctgatgact tttggggcca aaccttacga tgggatccca
     6121 gcccgggaga tccctgacct gctggaaaag ggggagcggc tgccccagcc ccccatctgc
     6181 accattgatg tctacatgat catggtcaaa tgttggatga ttgactctga atgtcggcca
     6241 agattccggg agttggtgtc tgaattctcc cgcatggcca gggaccccca gcgctttgtg
     6301 gtcatccaga atgaggactt gggcccagcc agtcccttgg acagcacctt ctaccgctca
     6361 ctgctggagg acgatgacat gggggacctg gtggatgctg aggagtatct ggtaccccag
     6421 cagggcttct tctgtccaga ccctgccccg ggcgctgggg gcatggtcca ccacaggcac
     6481 cgcagctcat ctaccaggag tggcggtggg gacctgacac tagggctgga gccctctgaa
     6541 gaggaggccc ccaggtctcc actggcaccc tccgaagggg ctggctccga tgtatttgat
     6601 ggtgacctgg gaatgggggc agccaagggg ctgcaaagcc tccccacaca tgaccccagc
     6661 cctctacagc ggtacagtga ggaccccaca gtacccctgc cctctgagac tgatggctac
     6721 gttgcccccc tgacctgcag cccccagcct gaatatgtga accagccaga tgttcggccc
     6781 cagccccctt cgccccgaga gggccctctg cctgctgccc gacctgctgg tgccactctg
     6841 gaaaggccca agactctctc cccagggaag aatggggtcg tcaaagacgt ttttgccttt
     6901 gggggtgccg tggagaaccc cgagtacttg acaccccagg gaggagctgc ccctcagccc
     6961 caccctcctc ctgccttcag cccagccttc gacaacctct attactggga ccaggaccca
     7021 ccagagcggg gggctccacc cagcaccttc aaagggacac ctacggcaga gaacccagag
     7081 tacctgggtc tggacgtgcc agtgtgacca actttcttgt acaaagtggt gatgtaggaa
     7141 ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7201 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7321 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7381 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7441 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7501 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7561 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7621 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7681 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7741 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7801 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7861 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7921 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7981 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     8041 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     8101 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     8161 tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     8221 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     8281 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     8341 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     8401 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     8461 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     8521 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     8581 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     8641 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     8701 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     8761 tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat
     8821 taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg
     8881 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
