LOCUS       sequence-156756-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to
            Geminin peptide and is degraded during G0/G1 phases of the 
            cell-cycle to minimize indels caused by non-homologous end joining.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, 
            Elefanty AG, Stanley EG
  TITLE     A Cas9 Variant for Efficient Generation of Indel-Free Knockin or 
            Gene-Corrected Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 
            10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
  PUBMED    27499201
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2016.07.001"; journalName: "Stem Cell Reports"; 
            date: "2016-09-13- 13"; volume: "7"; issue: "3"; pages: "508-17"
COMMENT     Sequence Label: pDNR-SpCas9-Gem
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tatagggcgc tagaggatcg aacccttgcc accatggaca agaagtactc cattgggctc
       61 gatatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc
      121 aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc
      181 gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg
      241 cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag
      301 atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag
      361 gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat
      421 gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct
      481 gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc
      541 atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt
      601 cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa
      661 gcaatcctga gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc
      721 cctggggaga agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc
      781 cccaacttta aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac
      841 acctacgatg atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt
      901 tttttggcgg caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac
      961 acggagatca ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac
     1021 caagacttga ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa
     1081 attttcttcg atcagtctaa aaa
//
