LOCUS       sequence-156758-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to silence target human FASN expression via RNA interference 
            (3rd gen lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     Elizabeth Stoll plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label:  pLKO.1 puro-humanU6-shRNA FASN
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    117..234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(332..580)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(390..409)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(560..580)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(878..922)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc
       61 ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa
      121 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
      181 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
      241 atgaatactg ccatttgtct cgaggtcgag aattcaaaaa cgagagcacc tttgatgaca
      301 tctcgagatg tcatcaaagg tgctctcgac cggtgtttcg tcctttccac aagatatata
      361 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      421 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      481 ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca gccttgtatc
      541 gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtgaagggg gcggccgctc
      601 gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg gatctgtctc
      661 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
      721 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
      781 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
      841 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
      901 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtg
//
