LOCUS       sequence-156763-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Human WT SIRT5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Kumar S, Lombard DB
  TITLE     Generation and Purification of Catalytically Active Recombinant 
            Sirtuin5 (SIRT5) Protein.
  JOURNAL   Methods Mol Biol. 2016;1436:241-57. doi: 
            10.1007/978-1-4939-3667-0_16.
  PUBMED    27246219
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1436:241-57. doi"; pages: " 
            10.1007/978-1-4939-3667-0_16"
COMMENT     Sequence Label: SIRT5-pET15b
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgagttca agtatggcag
      121 attttcgaaa gttttttgca aaagcaaagc acatagtcat catctcagga gctggtgtta
      181 gtgcagaaag tggtgttccg accttcagag gagctggagg ttattggaga aaatggcaag
      241 cccaggacct ggcgactccc ctggcctttg cccacaaccc gtcccgggtg tgggagttct
      301 accactaccg gcgggaggtc atggggagca aggagcccaa cgccgggcac cgcgccatag
      361 ccgagtgtga gacccggctg ggcaagcagg gccggcgagt cgtggtcatc acccagaaca
      421 tcgatgagct gcaccgcaag gctggcacca agaaccttct ggagatccat ggtagcttat
      481 ttaaaactcg atgtacctct tgtggagttg tggctgagaa ttacaagagt ccaatttgtc
      541 cagctttatc aggaaaaggt gctccagaac ctggaactca agatgccagc atcccagttg
      601 agaaacttcc ccggtgtgaa gaggcaggct gcgggggctt gctgcgacct cacgtcgtgt
      661 ggtttggaga aaacctggat cctgccattc tggaggaggt tgacagagag ctcgcccact
      721 gtgatttatg tctagtggtg ggcacttcct ctgtggtgta cccagcagcc atgtttgccc
      781 cccaggtggc tgccaggggc gtgccagtgg ctgaatttaa cacggagacc accccagcta
      841 cgaacagatt caggtttcat ttccagggac cctgtggaac gactcttcct gaagcccttg
      901 cctgtcatga aaatgaaact gtttcttaac tcgaggatcc ggctgctaa
//
