LOCUS       sequence-156764-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Human WT SIRT5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Kumar S, Lombard DB
  TITLE     Generation and Purification of Catalytically Active Recombinant 
            Sirtuin5 (SIRT5) Protein.
  JOURNAL   Methods Mol Biol. 2016;1436:241-57. doi: 
            10.1007/978-1-4939-3667-0_16.
  PUBMED    27246219
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1436:241-57. doi"; pages: " 
            10.1007/978-1-4939-3667-0_16"
COMMENT     Sequence Label: SIRT5-pET15b
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(864..881)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(891..908)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             927..949
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    964..988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctcg agttaagaaa cagtttcatt ttcatgacag
       61 gcaagggctt caggaagagt cgttccacag ggtccctgga aatgaaacct gaatctgttc
      121 gtagctgggg tggtctccgt gttaaattca gccactggca cgcccctggc agccacctgg
      181 ggggcaaaca tggctgctgg gtacaccaca gaggaagtgc ccaccactag acataaatca
      241 cagtgggcga gctctctgtc aacctcctcc agaatggcag gatccaggtt ttctccaaac
      301 cacacgacgt gaggtcgcag caagcccccg cagcctgcct cttcacaccg gggaagtttc
      361 tcaactggga tgctggcatc ttgagttcca ggttctggag caccttttcc tgataaagct
      421 ggacaaattg gactcttgta attctcagcc acaactccac aagaggtaca tcgagtttta
      481 aataagctac catggatctc cagaaggttc ttggtgccag ccttgcggtg cagctcatcg
      541 atgttctggg tgatgaccac gactcgccgg ccctgcttgc ccagccgggt ctcacactcg
      601 gctatggcgc ggtgcccggc gttgggctcc ttgctcccca tgacctcccg ccggtagtgg
      661 tagaactccc acacccggga cgggttgtgg gcaaaggcca ggggagtcgc caggtcctgg
      721 gcttgccatt ttctccaata acctccagct cctctgaagg tcggaacacc actttctgca
      781 ctaacaccag ctcctgagat gatgactatg tgctttgctt ttgcaaaaaa ctttcgaaaa
      841 tctgccatac ttgaactcat atggctgccg cgcggcacca ggccgctgct gtgatgatga
      901 tgatgatggc tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
      961 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtat
//
