LOCUS       sequence-156766-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Human catalytic mutant SIRT5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kumar S, Lombard DB
  TITLE     Generation and Purification of Catalytically Active Recombinant 
            Sirtuin5 (SIRT5) Protein.
  JOURNAL   Methods Mol Biol. 2016;1436:241-57. doi: 
            10.1007/978-1-4939-3667-0_16.
  PUBMED    27246219
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1436:241-57. doi"; pages: " 
            10.1007/978-1-4939-3667-0_16"
COMMENT     Sequence Label: SIRT5H158Y-pET15b
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgag ttcaagtatg gcagattttc
      121 gaaagttttt tgcaaaagca aagcacatag tcatcatctc aggagctggt gttagtgcag
      181 aaagtggtgt tccgaccttc agaggagctg gaggttattg gagaaaatgg caagcccagg
      241 acctggcgac tcccctggcc tttgcccaca acccgtcccg ggtgtgggag ttctaccact
      301 accggcggga ggtcatgggg agcaaggagc ccaacgccgg gcaccgcgcc atagccgagt
      361 gtgagacccg gctgggcaag cagggccggc gagtcgtggt catcacccag aacatcgatg
      421 agctgcaccg caaggctggc accaagaacc ttctggagat ctatggtagc ttatttaaaa
      481 ctcgatgtac ctcttgtgga gttgtggctg agaattacaa gagtccaatt tgtccagctt
      541 tatcaggaaa aggtgctcca gaacctggaa ctcaagatgc cagcatccca gttgagaaac
      601 ttccccggtg tgaagaggca ggctgcgggg gcttgctgcg acctcacgtc gtgtggtttg
      661 gagaaaacct ggatcctgcc attctggagg aggttgacag agagctcgcc cactgtgatt
      721 tatgtctagt ggtgggcact tcctctgtgg tgtacccagc agccatgttt gccccccagg
      781 tggctgccag gggcgtgcca gtggctgaat ttaacacgga gaccacccca gctacgaaca
      841 gattcaggtt tcatttccag ggaccctgtg gaacgactct tcctgaagcc cttgcctgtc
      901 atgaaaatga aactgtttct taactcgagg atccg
//
