LOCUS       sequence-156769-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter to quantify the ratio of two alternative 
            splicing patterns..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Orengo JP, Bundman D, Cooper TA
  TITLE     A bichromatic fluorescent reporter for cell-based screens of 
            alternative splicing.
  JOURNAL   Nucleic Acids Res. 2006;34(22):e148. Epub 2006 Nov 16.
  PUBMED    17142220
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(22)"; pages: "e148. Epub 2006
            Nov 16"
COMMENT     Sequence Label: FRE5
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             131..151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(186..188,189..863)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(366..386)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     584..603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     778..801
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttc catggattac aaggatgacg atgacaaggg
      121 ggtacctgcc ccaaaaaaaa aacgcaaagt ggaggaccca gtaccaggat ccaatcgata
      181 ccggtgtgcg ctcctccaag aacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
      241 agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 agggccacaa caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
      361 acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca
      421 tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      481 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggctgcttca
      541 tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga
      601 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
      661 gcgagatcca caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt
      721 ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gactccaagc
      781 tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcaccgagg
      841 gccgccacca cctgttcctg tagaccgcgg tgtgagcaag ggcgaggagc tgttcaccgg
      901 ggtggtgccc atcctggtc
//
