LOCUS       sequence-156771-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from FRE5. A two nucleotide insertion to shift the reading 
            frame to dsRED..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Orengo JP, Bundman D, Cooper TA
  TITLE     A bichromatic fluorescent reporter for cell-based screens of 
            alternative splicing.
  JOURNAL   Nucleic Acids Res. 2006;34(22):e148. Epub 2006 Nov 16.
  PUBMED    17142220
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(22)"; pages: "e148. Epub 2006
            Nov 16"
COMMENT     Sequence Label: FRE5Cf
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             143..163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(380..400)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     598..617
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     792..815
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tccatggatt acaaggatga
      121 cgatgacaag ggggtacctg ccccaaaaaa aaaacgcaaa gtggaggacc cagtaccagg
      181 atccaatcgc gataccggtg tgcgctcctc caagaacgtc atcaaggagt tcatgcgctt
      241 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      301 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct
      361 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
      421 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
      481 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      541 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
      601 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
      661 cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct
      721 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
      781 cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
      841 cgagcgcacc gagggccgcc accacctgt
//
