LOCUS       sequence-156773-        8519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Bouyer D, Geier F, Kragler F, Schnittger A, Pesch M, Wester K, 
            Balkunde R, Timmer J, Fleck C, Hulskamp M
  TITLE     Two-dimensional patterning by a trapping/depletion mechanism: the 
            role of TTG1 and GL3 in Arabidopsis trichome formation.
  JOURNAL   PLoS Biol. 2008 Jun 10;6(6):e141. doi: 10.1371/journal.pbio.0060141.
  PUBMED    18547143
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2008-06-10"; volume: "6(6):e141. doi"; pages: " 
            10.1371/journal.pbio.0060141"
COMMENT     Sequence Label: pAM-PAT-ProTTG1 GW
FEATURES             Location/Qualifiers
     source          1..8519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..26
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(120..372)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(392..943)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(985..1164)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     RBS             1851..1859
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    3504..3628
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3653..3658,3659..3676,3677..3683)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3737..4396
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3803..3822)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4738..5043
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4922..4941
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5084..5208)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5579..5603
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5715..6424
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6485..6589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6590..6658,6659..7450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6808..6827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7621..8209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8110..8129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
       61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
      121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
      421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
      481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
      541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
      601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
      661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
      721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
      781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
      841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
      901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
      961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
     1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
     1261 ccgccctatt aaaaaacaaa aataacaatt acacaatcac tcgtcttgaa caaacaggtc
     1321 tagaaacatc atcccgtaag atttcatccg cacaccggag aacataaaca agagcataaa
     1381 agcttaaaga caagcatagt ttgttaacat gtccgtaaaa tgattagcct ctctatatgt
     1441 gaaacacggt caatctagtt tttcgataaa aaactatagc gcaaacgtac tggaaatgat
     1501 agcagatgag agtcccataa ccttgtcttc aaaatctcaa ccaccattta ccacaaatat
     1561 ggggatgaaa acaggcaaac ggtctcatac gttgtaaata agcattctta atgtcaagtt
     1621 ggtagatagg ccataaaata agcatcctta tgtttagcgc atagcctcca caccattcac
     1681 cctcctcatt acgtatcaga ccaccaccag ccgcgagtct cgaattgcca taaaataccc
     1741 catcagtatt taatttaaac cagcccatag acgagataag ccattttatc agcttctcaa
     1801 cccgaccagc cctttgtgtt gcctttccgc tactagctct cgcctccaat acctccttag
     1861 ctaactctct tatgaaccgc accctattct tccatacttt attctcccca aaaactagct
     1921 aattgaatta ccaacatttg atcaagataa tatactaggt agctaattaa tgagctcatt
     1981 tttttttgtc gtcaatgggc taatttatta attacagtat gaactattga ctattattct
     2041 aaataagtga atatcacgag tatgtacgaa ttattggatg tatctatttg tattgattga
     2101 tgtaatatca aatagtatga atttggagta aacgtgggtt tggggttgaa gcaggtaggg
     2161 catgtcaaag tagggcgtct ttcgttatgt ccctttcctc taaatttgaa cctctgtcat
     2221 tgtacagaaa aatcgtaata acccataaat gtgttttaaa aaacattatt tcgagttttc
     2281 tacacatatt ctagtcatgt ttaatttgaa tcttttctta tttaagtaag ctttagacat
     2341 ttttaaccta agttttcttc tcccttcata aattttgaga tctatataat gttcttacat
     2401 tttggatcaa gatcttcata ttctcattcc aattagtaaa agattttttc accttttaat
     2461 ctcttatctt ttatttatat tctttagtta tgtttatgct tttcatcata tttagtggtt
     2521 agtttttatt atttatttat tgattcatga cttatgctag attatgataa gaatttatgt
     2581 taccacttga taaatcctcc atttgacatg tgtttaatgc tagatttata ttgtctccaa
     2641 atttacaact tcgatgtctt atgataaatg ccaacaacca aatttcagat aaagattagc
     2701 agactaacta agcttattat tcacttgcaa ggtggagtga tgttgaaaga accctcacag
     2761 acacgtcatt gggaagacta aatctctttt tagcacgtta cacctttgag atcgcgttta
     2821 ttccatatgg agagagagca acaatacgag acatggagag gcaccattac cgccggcgca
     2881 actgcttcca aatattgaca aacaaatttg aatctggatc ttctctattc gtgaacaagg
     2941 agatagaagc tacgatgaat gcatggaagc ttggtttgct ttaatataaa cactaaaggg
     3001 gagtagaact ttcttgaaaa attgtatgca aattatttac cgaatgttaa aagctttttt
     3061 cgaataaatt ttacattttc ttaataataa taataaaaaa ggattgttga ttatcttaat
     3121 cacaaacaat ttattttagc tgaattagac aattgttagt aaaatgatta gagtgtcaca
     3181 tattaatgtt gttagtgttt catgtcatcc tagtgatcca ataattaggc cattctatag
     3241 ctcttaacgt taaaataaaa ggcccattat ctgaatatac agaagcccat tatcaataga
     3301 tacattaaaa gatactgatt aatccagagg gtttatatct acgccgtctc cattgattat
     3361 ttctccgtct cttgaaaaat ccgactgaca ctgacctcaa aactctcctc tcactttcgt
     3421 cgtgaagaag ccaaatctcg aatcgaatca gcaccacaca tttccttcgg tttgggtcga
     3481 gaaagaggat ccacctgagg atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     3541 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     3601 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     3661 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     3721 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     3781 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     3841 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     3901 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     3961 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4021 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     4081 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     4141 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     4201 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     4261 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     4321 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     4381 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
     4441 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     4501 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     4561 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     4621 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     4681 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     4741 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     4801 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     4861 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     4921 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     4981 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     5041 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     5101 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     5161 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tcctccgttc
     5221 catgggctag aagcttgttg agaattcatc gcccgggact gggttaactt ccgatcgatt
     5281 ctagactagt tctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     5341 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     5401 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     5461 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgaccgg gcggccgcca
     5521 ccgcggtgga gggggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     5581 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     5641 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgtacat caccgacgag
     5701 caaggcaaga ccgagcgcct ttccgacgct caccgggctg gttgccctcg ccgctgggct
     5761 ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca
     5821 ccggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg
     5881 cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg
     5941 ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat
     6001 tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt
     6061 gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc
     6121 agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa
     6181 aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac
     6241 ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt
     6301 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
     6361 ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg
     6421 gcagcgctgg cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc
     6481 ccgacaagct acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7741 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg actctagcta
     8281 gaggatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
     8341 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
     8401 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
     8461 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctg
//
