LOCUS       sequence-156776-       15960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses YFP-Ezh2 fusion proteins in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15960)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-YFP-Ezh2
FEATURES             Location/Qualifiers
     source          1..15960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3027..3036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3033..3035,3036..3038,3039..3749)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3078..3099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3339..3358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3686..3707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6029..7240
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7077..7096
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7259..7966
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    7989..8561
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8156..8173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8383..8402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8573..9367
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8573..9367
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8627..8646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9237..9256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9812..9832
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9931..10519
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9984..10004)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10402..10413)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10726..10959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11060..11077)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11066..11290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11336..11764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11423..11442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11633..11654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11773..11793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11778..12107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11958..12093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12020..12039
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    13315..13436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13485..13501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13485..13501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13498..13520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13509..13525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13533..13539,13540..13557,13558..13563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13578..13599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13716..13733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13887..14475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13967..13986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14646..15437,15438..15506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15269..15288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15507..15611)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    15659..15793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtcgt
     3001 ggaattcgtt aactacgtac tcgagggcca ccatggtgag caagggcgag gagctgttca
     3061 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     3241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3481 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3601 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     3661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3721 tcactctcgg catggacgag ctgtacaagg cgaattcgat acgtatcgat ggcgcgccca
     3781 tgggccagac tgggaagaaa tctgagaagg gaccagtttg ttggcggaag cgtgtaaaat
     3841 cagagtacat gcgactgaga cagctcaaga ggttcagacg agctgatgaa gtaaagagta
     3901 tgtttagttc caatcgtcag aaaattttgg aaagaacgga aatcttaaac caagaatgga
     3961 aacagcgaag gatacagcct gtgcacatcc tgacttctgt gagctcattg cgcgggacta
     4021 gggagtgttc ggtgaccagt gacttggatt ttccaacaca agtcatccca ttaaagactc
     4081 tgaatgcagt tgcttcagta cccataatgt attcttggtc tcccctacag cagaatttta
     4141 tggtggaaga tgaaactgtt ttacataaca ttccttatat gggagatgaa gttttagatc
     4201 aggatggtac tttcattgaa gaactaataa aaaattatga tgggaaagta cacggggata
     4261 gagaatgtgg gtttataaat gatgaaattt ttgtggagtt ggtgaatgcc cttggtcaat
     4321 ataatgatga tgacgatgat gatgatggag acgatcctga agaaagagaa gaaaagcaga
     4381 aagatctgga ggatcaccga gatgataaag aaagccgccc acctcggaaa tttccttctg
     4441 ataaaatttt tgaagccatt tcctcaatgt ttccagataa gggcacagca gaagaactaa
     4501 aggaaaaata taaagaactc accgaacagc agctcccagg cgcacttcct cctgaatgta
     4561 cccccaacat agatggacca aatgctaaat ctgttcagag agagcaaagc ttacactcct
     4621 ttcatacgct tttctgtagg cgatgtttta aatatgactg cttcctacat ccttttcatg
     4681 caacacccaa cacttataag cggaagaaca cagaaacagc tctagacaac aaaccttgtg
     4741 gaccacagtg ttaccagcat ttggagggag caaaggagtt tgctgctgct ctcaccgctg
     4801 agcggataaa gaccccacca aaacgtccag gaggccgcag aagaggacgg cttcccaata
     4861 acagtagcag gcccagcacc cccaccatta atgtgctgga atcaaaggat acagacagtg
     4921 atagggaagc agggactgaa acggggggag agaacaatga taaagaagaa gaagagaaga
     4981 aagatgaaac ttcgagctcc tctgaagcaa attctcggtg tcaaacacca ataaagatga
     5041 agccaaatat tgaacctcct gagaatgtgg agtggagtgg tgctgaagcc tcaatgttta
     5101 gagtcctcat tggcacttac tatgacaatt tctgtgccat tgctaggtta attgggacca
     5161 aaacatgtag acaggtgtat gagtttagag tcaaagaatc tagcatcata gctccagctc
     5221 ccgctgagga tgtggatact cctccaagga aaaagaagag gaaacaccgg ttgtgggctg
     5281 cacactgcag aaagatacag ctgaaaaagg acggctcctc taaccatgtt tacaactatc
     5341 aaccctgtga tcatccacgg cagccttgtg acagttcgtg cccttgtgtg atagcacaaa
     5401 atttttgtga aaagttttgt caatgtagtt cagagtgtca aaaccgcttt ccgggatgcc
     5461 gctgcaaagc acagtgcaac accaagcagt gcccgtgcta cctggctgtc cgagagtgtg
     5521 accctgacct ctgtcttact tgtggagccg ctgaccattg ggacagtaaa aatgtgtcct
     5581 gcaagaactg cagtattcag cggggctcca aaaagcatct attgctggca ccatctgacg
     5641 tggcaggctg ggggattttt atcaaagatc ctgtgcagaa aaatgaattc atctcagaat
     5701 actgtggaga gattatttct caagatgaag ctgacagaag agggaaagtg tatgataaat
     5761 acatgtgcag ctttctgttc aacttgaaca atgattttgt ggtggatgca acccgcaagg
     5821 gtaacaaaat tcgttttgca aatcattcgg taaatccaaa ctgctatgca aaagttatga
     5881 tggttaacgg tgatcacagg ataggtattt ttgccaagag agccatccag actggcgaag
     5941 agctgttttt tgattacaga tacagccagg ctgatgccct gaagtatgtc ggcatcgaaa
     6001 gagaaatgga aatcccttga cgacgcgtgg cctccgcgcc gggttttggc gcctcccgcg
     6061 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     6121 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     6181 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     6241 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     6301 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     6361 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     6421 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     6481 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     6541 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     6601 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     6661 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     6721 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     6781 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     6841 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     6901 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     6961 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     7021 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     7081 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     7141 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     7201 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gctagcggat ccgccaccat
     7261 gtctaggctg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     7321 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     7381 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     7441 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     7501 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     7561 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     7621 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     7681 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     7741 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     7801 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     7861 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     7921 agccgatgcc cttgacgatt ttgaccttga catgctcccc gggtaaggat ccgcggccgc
     7981 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     8041 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     8101 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     8161 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     8221 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     8281 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     8341 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     8401 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     8461 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     8521 aaccacgggg acgtggtttt cctttgaaaa acacgataat acccctgcag gtatgattga
     8581 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     8641 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     8701 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     8761 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     8821 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     8881 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     8941 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     9001 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     9061 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     9121 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     9181 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     9241 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     9301 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     9361 cttctgatga tcgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     9421 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     9481 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     9541 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg
     9601 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     9661 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9721 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg
     9781 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9841 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9901 aagcccggtg cctgagttcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     9961 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    10021 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    10081 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    10141 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    10201 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10261 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10321 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10381 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10441 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10501 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
    10561 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
    10621 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
    10681 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
    10741 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    10801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
    10981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
    11041 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11101 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    11161 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    11221 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    11281 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    11341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    11581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    11641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    11701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    11761 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    11821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    11881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    11941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    12001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    12061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    12121 cttgtatatc cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga
    12181 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
    12241 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
    12301 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
    12361 cggccgcgct cccgattccg gaagtgcttg acattgggga attaattcag cgagagcctg
    12421 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
    12481 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
    12541 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
    12601 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
    12661 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
    12721 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
    12781 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
    12841 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
    12901 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
    12961 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    13021 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    13081 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    13141 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat
    13201 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    13261 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    13321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    13381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    13441 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    13501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    13561 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    13621 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    13681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    13741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    13801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    13861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    13921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    13981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    14041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    14101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    14161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    14221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    14281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    14341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    14401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    14461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    14521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    14581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    14641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    14701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    14761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    14821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    14881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    14941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    15001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    15061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    15121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    15181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    15241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    15301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    15361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    15421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    15481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    15541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    15601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    15661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    15721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    15781 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    15841 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    15901 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
