LOCUS       sequence-156788-       14347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses Halotag-Cbx6 fusion proteins in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14347)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-Cbx6
FEATURES             Location/Qualifiers
     source          1..14347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3014..3904
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        5165..6376
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6213..6232
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6395..7102
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    7125..7697
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7292..7309)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7519..7538
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7700..8302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8199..8219
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8318..8906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8371..8391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8789..8800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9113..9346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9447..9464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9453..9677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9723..10151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9810..9829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10020..10041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10160..10180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10165..10494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10345..10480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10407..10426
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11702..11823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11872..11888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11872..11888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11885..11907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11896..11912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11920..11926,11927..11944,11945..11950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11965..11986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12103..12120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12274..12862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12354..12373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13033..13824,13825..13893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13656..13675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13894..13998)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14046..14180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
     3061 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     3121 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     3181 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     3241 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     3301 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     3361 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     3421 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     3481 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     3541 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     3601 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     3661 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     3721 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     3781 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     3841 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     3901 cggcgcgaat tcctgcagga tggagctgtc tgcagtgggc gagcgggtct tcgcggccga
     3961 atccatcatc aaacggcgga tccgaaaggg acgcatcgag tacctggtga aatggaaggg
     4021 gtgggcgatc aagtacagca cttgggagcc cgaggagaac atcctggact cgcggctcat
     4081 tgcagccttc gaacaaaagg agagggagcg tgagctgtat gggcccaaga agaggggacc
     4141 caaacccaaa actttcctcc tgaaggcgcg ggcccaggcc gaggccctcc gcatcagtga
     4201 tgtgcatttc tctgtcaagc cgagcgccag tgcctcctcg cccaagctgc actccagcgc
     4261 agccgtgcac cggctcaaga aggacatccg ccgctgccac cgtatgtccc gccgtcccct
     4321 gccccgcccg gacccgcagg ggggcagccc cggactgcgc ccgcccattt cgcccttctc
     4381 ggagacggtg cgcatcatca accgcaaggt gaagccgcgg gagcccaagc ggaaccgcat
     4441 catcctgaac ctgaaggtga tcgacaaggg cgctggcggc gggggcgccg ggcagggggc
     4501 cggggcgctg gcccgcccca aagtcccctc gcggaaccgc gttataggca agagcaagaa
     4561 gttcagcgag agcgtcctgc gtacacagat ccgccacatg aagttcggcg cctttgcgct
     4621 gtacaagcct ccgcccgccc ccctggtagc cccgtccccc ggcaaggctg aggcctcagc
     4681 cccgggccct gggctacttc tggccgcccc cgccgccccc tacgacgccc gcagctctgg
     4741 ctcctccggc tgcccctcgc ctacaccaca gtcctctgac cccgacgaca cgccccccaa
     4801 gctcctcccc gagaccgtga gcccatccgc ccccagctgg cgcgagccgg aggtgctcga
     4861 cctgtccctc cctcccgagt cggcagccac cagcaagcgg gcaccgcctg aggtcacagc
     4921 tgctgccggc ccggcacctc ccacggcccc tgagcccgcc ggtgcctcct ccgagcccga
     4981 ggctggggac tggcgccccg agatgtcacc ctgctccaat gtggtcgtca ccgatgtcac
     5041 cagcaacctc ctgacggtca caatcaagga attctgcaac cctgaggatt tcgagaaggt
     5101 ggctgctggg gtagcaggcg ccgctggggg cggtggcagc attggggcga gcaagtgaac
     5161 gcgtggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     5221 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     5281 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     5341 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     5401 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     5461 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     5521 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
     5581 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
     5641 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
     5701 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
     5761 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
     5821 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
     5881 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
     5941 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg
     6001 tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg
     6061 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct
     6121 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg
     6181 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc
     6241 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg
     6301 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
     6361 ggcttttttg ttagacgcta gcggatccgc caccatgtct aggctggaca agagcaaagt
     6421 cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa
     6481 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     6541 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     6601 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc
     6661 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     6721 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     6781 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     6841 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     6901 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     6961 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac
     7021 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgattttga
     7081 ccttgacatg ctccccgggt aaggatccgc ggccgcaaat tccgcccctc tccctccccc
     7141 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     7201 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     7261 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     7321 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     7381 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     7441 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     7501 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     7561 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     7621 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7681 tgaaaaacac gataatacca tggccaccga gtacaagccc acggtgcgcc tcgccacccg
     7741 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7801 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7861 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7921 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggctc
     7981 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8041 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8101 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8161 cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8221 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8281 gacccgcaag cccggtgcct gagttcgcgt ctggaacaat caacctctgg attacaaaat
     8341 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8401 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8461 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8521 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8581 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8641 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8701 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8761 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8821 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8881 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     8941 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     9001 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     9061 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     9121 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     9181 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9241 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9301 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     9361 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     9421 tgacattgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     9481 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     9541 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     9601 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     9661 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     9721 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     9961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10021 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    10081 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    10141 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    10201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    10261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    10441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    10501 ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc
    10561 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
    10621 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
    10681 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
    10741 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt aattcagcga
    10801 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
    10861 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
    10921 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
    10981 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
    11041 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
    11101 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
    11161 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
    11221 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
    11281 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
    11341 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
    11401 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
    11461 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
    11521 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct
    11581 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    11641 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    11701 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11761 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11821 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    11881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    11941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    12001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    12061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    12121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    12181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    12241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    12301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    12361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    12421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    12481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    12541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    12601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    12661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    12721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    12781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    12841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    12901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    12961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    13021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    13081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    13141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    13201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    13261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    13321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    13381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    13441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    13501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    13561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    13621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    13681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    13741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    13801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    13861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    13921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    13981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    14041 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    14101 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    14161 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    14221 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    14281 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    14341 tagtagt
//
