LOCUS       sequence-156792-       14289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses halotag Cbx8fusion proteins in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14289)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ(M)-HT-Cbx8
FEATURES             Location/Qualifiers
     source          1..14289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3014..3904
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        5107..6318
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6155..6174
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6337..7044
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    7067..7639
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7234..7251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7461..7480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7642..8244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8141..8161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8260..8848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8313..8333)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8731..8742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9055..9288
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9389..9406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9395..9619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9665..10093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9752..9771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9962..9983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10102..10122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10107..10436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10287..10422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10349..10368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11644..11765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11814..11830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11814..11830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11827..11849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11838..11854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11862..11868,11869..11886,11887..11892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11907..11928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12045..12062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12216..12804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12296..12315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12975..13766,13767..13835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13598..13617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13836..13940)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13988..14122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
     3061 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     3121 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     3181 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     3241 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     3301 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     3361 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     3421 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     3481 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     3541 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     3601 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     3661 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     3721 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     3781 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     3841 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     3901 cggcgcgaat tcacttgcta tggagctttc agcggtgggg gagcgggtgt tcgcggccga
     3961 agccctcctg aagcggcgca tacggaaagg acgcatggaa tacctcgtga aatggaaggg
     4021 atggtcgcag aagtacagca catgggaacc ggaggaaaac atcctggatg ctcgcttgct
     4081 cgcagccctt gaggaaaggg aaagagagat ggagctctat ggccccaaaa agcgtggacc
     4141 caagcccaaa accttcctcc tcaaagcgca ggccaaggca aaggccaaaa cttacgagtt
     4201 tcgaagtgac tcagccaggg gcatccggat cccctaccct ggccgctcgc cccaggacct
     4261 ggcctccact tcccgggccc gggagggcct tcgaaacatg ggtttgtccc cgccagcgag
     4321 cagcaccagc accagcagca cctgccgcgc agaggcccct cgggaccggg accgagaccg
     4381 ggatagggac cgggagcggg atcgagaaag ggagagggag cgagagaggg agcgggaacg
     4441 tgagagggaa cgagagcggg gtaccagcag agtggatgac aagcccagct caccggggga
     4501 cagctcgaag aagcgaggcc ccaagccccg gaaggagctc ccggacccct cacagaggcc
     4561 cttaggcgaa cccagcgccg gcctcggaga gtacctcaag ggcaggaagc tggacgacac
     4621 cccttccggg gcaggaaagt ttccagccgg ccacagtgtg atccagctgg cccgaagaca
     4681 ggactcggac ctggtgcagt gtggtgtgac cagccctagc tcagctgagg ccacgggcaa
     4741 actggctgtg gacaccttcc cggccagggt gataaagcac agggctgcct tcctggaggc
     4801 caaaggccag ggtgccctag atcccaatgg cacccgggtc cgacatggct caggcccccc
     4861 cagctctggg gggggcctgt accgggacat gggggcccag gggggaaggc cctccctcat
     4921 cgccaggatc cctgtggcca gaatcctggg ggacccggag gaagagtcct ggagcccctc
     4981 cctgactaac ctggagaagg tggtggtcac ggacgtgacc tcaaactttt tgaccgtcac
     5041 cattaaggaa agtaacacgg accaaggctt ttttaaagag aaaagatgag gcgcgccccg
     5101 acgcgtggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     5161 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     5221 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     5281 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     5341 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     5401 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     5461 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     5521 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     5581 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     5641 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     5701 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     5761 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     5821 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     5881 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     5941 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     6001 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     6061 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     6121 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     6181 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     6241 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     6301 ttggcttttt tgttagacgc tagcggatcc gccaccatgt ctaggctgga caagagcaaa
     6361 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     6421 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     6481 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     6541 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     6601 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     6661 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     6721 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     6781 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     6841 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     6901 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     6961 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgatttt
     7021 gaccttgaca tgctccccgg gtaaggatcc gcggccgcaa attccgcccc tctccctccc
     7081 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     7141 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     7201 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     7261 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     7321 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     7381 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     7441 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     7501 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     7561 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     7621 tttgaaaaac acgataatac catggccacc gagtacaagc ccacggtgcg cctcgccacc
     7681 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7741 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7921 tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8101 cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8221 atgacccgca agcccggtgc ctgagttcgc gtctggaaca atcaacctct ggattacaaa
     8281 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8341 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8401 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8461 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8521 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8581 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8641 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     8701 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8761 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8821 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     8881 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     8941 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     9001 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     9061 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9181 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9301 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     9361 cttgacattg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9421 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9481 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9541 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     9601 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     9661 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10081 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10201 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10261 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10321 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
    10381 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10441 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa aagcctgaac
    10501 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
    10561 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
    10621 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
    10681 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttaattcagc
    10741 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
    10801 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
    10861 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    10921 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    10981 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    11041 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    11101 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    11161 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    11221 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
    11281 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    11341 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    11401 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    11461 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgtg
    11521 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    11581 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    11641 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11761 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    11821 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    11881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    11941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    12001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    12061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    12121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    12181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    12301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    12361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    12421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    12481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    12541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    12661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    12721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    13021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    13081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    13141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    13201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    13261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    13321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    13381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    13441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    13501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    13561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    13621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    13681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    13741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    13981 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    14041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14101 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    14161 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    14221 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    14281 cttagtagt
//
