LOCUS       sequence-156805-       13682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses mutant Halotag-Cbx7-Delta-CD fusion 
            proteins in mammalian cells. Cbx7 chromodomain deletion deletion of 
            amino acids 1–62..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13682)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-Cbx7-Delta-CD
FEATURES             Location/Qualifiers
     source          1..13682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3014..3904
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        4500..5711
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5548..5567
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5730..6437
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    6460..7032
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6627..6644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6854..6873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7035..7637
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7534..7554
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7653..8241
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7706..7726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8124..8135)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8448..8681
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8782..8799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8788..9012
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9058..9486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9145..9164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9355..9376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9495..9515)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9500..9829
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9680..9815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9742..9761
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11037..11158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11207..11223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11207..11223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11220..11242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11231..11247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11255..11261,11262..11279,11280..11285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11300..11321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11438..11455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11609..12197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11689..11708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12368..13159,13160..13228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12991..13010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13229..13333)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13381..13515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
     3061 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     3121 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     3181 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     3241 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     3301 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     3361 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     3421 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     3481 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     3541 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     3601 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     3661 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     3721 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     3781 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     3841 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     3901 cggcgcgaat tcacttgcta gagaccgagc atcggggtat aggaagagag gtccgaaacc
     3961 caagcggctt ctgctgcagc ggctgtacag catggacctg cggagctccc acaaggccaa
     4021 gggcaaggag aagctctgct tctccctgac gtgcccactc ggcagcggga gccctgaggg
     4081 ggtggtcaag gcgggggcac ctgagctggt ggacaagggc cccttggtgc ccaccctgcc
     4141 cttcccgctc cgcaagcccc gaaaggccca caagtacctg cggctctcgc gcaagaagtt
     4201 cccgccccgc gggcccaacc tggagagcca cagccatcga cgggagctct tcctgcagga
     4261 gccaccggcc ccagacgtcc tgcaggcggc tggcgagtgg gagcctgctg cgcagccccc
     4321 tgaagaggag gcagatgccg acctggccga ggggccccct ccctggacac ctgcgctccc
     4381 ctcaagtgag gtgaccgtga ccgacatcac cgccaactcc atcaccgtca ccttccgcga
     4441 ggcccaggca gctgagggct tcttccgaga ccgcagtggg aagttctgag cggacgcgtg
     4501 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     4561 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     4621 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     4681 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     4741 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     4801 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     4861 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     4921 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     4981 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     5041 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     5101 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     5161 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     5221 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     5281 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     5341 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     5401 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     5461 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     5521 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     5581 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     5641 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     5701 ttttgttaga cgctagcgga tccgccacca tgtctaggct ggacaagagc aaagtcataa
     5761 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     5821 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     5881 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     5941 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     6001 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     6061 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     6121 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     6181 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     6241 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     6301 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     6361 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgat tttgaccttg
     6421 acatgctccc cgggtaagga tccgcggccg caaattccgc ccctctccct cccccccccc
     6481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6541 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6601 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6661 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6721 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6781 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6841 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     6901 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6961 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7021 aacacgataa taccatggcc accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7081 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7141 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     7201 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7261 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca
     7321 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7381 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7441 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg
     7501 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7561 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7621 gcaagcccgg tgcctgagtt cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     7681 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7741 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7801 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7861 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7921 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7981 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8041 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8101 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8161 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8221 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     8281 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     8341 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     8401 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     8461 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8521 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8581 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8641 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8701 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     8761 ttgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     8821 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     8881 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     8941 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     9001 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     9061 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9481 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     9781 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     9841 agcttgtata tccattttcg gatctgatca gcacgtgatg aaaaagcctg aactcaccgc
     9901 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     9961 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
    10021 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
    10081 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattaattc agcgagagcc
    10141 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    10201 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    10261 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    10321 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    10381 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    10441 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    10501 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    10561 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    10621 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    10681 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    10741 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    10801 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    10861 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag
    10921 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    10981 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11161 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11221 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11281 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11341 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11401 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    11521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    11581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    11641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    11701 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    11761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    11821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    11881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    12541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    12601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    12661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    12721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    12781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    12841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13321 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    13381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    13441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    13501 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    13561 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    13621 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    13681 gt
//
