LOCUS       sequence-156839-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-27a-3p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR-27a-3p
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..43)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(283..302)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     630..651
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaggttc
       61 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      121 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
      181 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      241 atgcccgaag gctacgtcca ggagcgtacc atcttcttca aggacgacgg caactacaag
      301 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      361 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
      421 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccca cttcaagatc
      481 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      541 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      601 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      661 gggatcactc tcggcatgga cgagctgtac aagtaakcga tcgctcgagc ggaacttagc
      721 cactgtgaam cgkgcggaac ttagyyactg wgaaggcmgc tggccgcaat aaaatatctt
      781 tattttcatt acatctgtgt gwtggttttt wgtgtgagga tctaaatgag tcttcggacc
      841 tcgcgggggc cgcttaagcg gtggytaggg tttgtctgac acgtkkcgaa gksggaagga
      901 acgaaacacw ctcattcgga ggcggctcga ggtttggtct tggtggccac gggcacgcaa
      961 aacagcgccg cgatcctctt aagcaccccc ccgccctccg tgtaggcggg kgtttggtcg
     1021 rcgnntgrta actgtcsggc cgctgaatcg ggmgggtcac gcgccctaga atrtgacctt
     1081 ttcggtctgm tcmcaaacct c
//
