LOCUS       sequence-156874-       10029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes TET1 under LITE2.0 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Lo CL, Choudhury SR, Irudayaraj J, Zhou FC
  TITLE     Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by 
            Optogenetics.
  JOURNAL   Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047.
  PUBMED    28181538
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-02-9"; volume: "7:42047. doi"; pages: " 
            10.1038/srep42047"
COMMENT     Sequence Label: TET1CD-CRY2-EGFP
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        231..1409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          462..1400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1357..1377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1416..1425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3597..3644
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5145..5165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5190..5339
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5361..5414
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5415..5417,5418..6131)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5415..5417,5418..6128)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5457..5478)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5718..5737)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6065..6086
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6156..6744
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6209..6229)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6627..6638)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6776..7252
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6975..6994)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   7292..7432
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7292..7421
                     /label=AAV2 ITR
     rep_origin      7507..7962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7594..7613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7804..7825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7979..7998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8098..8120
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8158..8176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8244..8348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8349..8417,8418..9209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8567..8586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9380..9968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9869..9888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga
      181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1381 gtttttttct tccatttcag gtgtcgtgag gtaccgccac catggaactg cccacctgca
     1441 gctgtcttga tcgagttata caaaaagaca aaggcccata ttatacacac cttggggcag
     1501 gaccaagtgt tgctgctgtc agggaaatca tggagaatag gtatggtcaa aaaggaaacg
     1561 caataaggat agaaatagta gtgtacaccg gtaaagaagg gaaaagctct catgggtgtc
     1621 caattgctaa gtgggtttta agaagaagca gtgatgaaga aaaagttctt tgtttggtcc
     1681 ggcagcgtac aggccaccac tgtccaactg ctgtgatggt ggtgctcatc atggtgtggg
     1741 atggcatccc tcttccaatg gccgaccggc tatacacaga gctcacagag aatctaaagt
     1801 catacaatgg gcaccctacc gacagaagat gcaccctcaa tgaaaatcgt acctgtacat
     1861 gtcaaggaat tgatccagag acttgtggag cttcattctc ttttggctgt tcatggagta
     1921 tgtactttaa tggctgtaag tttggtagaa gcccaagccc cagaagattt agaattgatc
     1981 caagctctcc cttacatgaa aaaaaccttg aagataactt acagagtttg gctacacgat
     2041 tagctccaat ttataagcag tatgctccag tagcttacca aaatcaggtg gaatatgaaa
     2101 atgttgcccg agaatgtcgg cttggcagca aggaaggtcg tcccttctct ggggtcactg
     2161 cttgcctgga cttctgtgct catccccaca gggacattca caacatgaat aatggaagca
     2221 ctgtggtttg taccttaact cgagaagata accgctcttt gggtgttatt cctcaagatg
     2281 agcagctcca tgtgctacct ctttataagc tttcagacac agatgagttt ggctccaagg
     2341 aaggaatgga agccaagatc aaatctgggg ccatcgaggt cctggcaccc cgccgcaaaa
     2401 aaagaacgtg tttcactcag cctgttcccc gttctggaaa gaagagggct gcgatgatga
     2461 cagaggttct tgcacataag ataagggcag tggaaaagaa acctattccc cgaatcaagc
     2521 ggaagaataa ctcaacaaca acaaacaaca gtaagccttc gtcactgcca accttaggga
     2581 gtaacactga gaccgtgcaa cctgaagtaa aaagtgaaac cgaaccccat tttatcttaa
     2641 aaagttcaga caacactaaa acttattcgc tgatgccatc cgctcctcac ccagtgaaag
     2701 aggcatctcc aggcttctcc tggtccccga agactgcttc agccacacca gctccactga
     2761 agaatgacgc aacagcctca tgcgggtttt cagaaagaag cagcactccc cactgtacga
     2821 tgccttcggg aagactcagt ggtgccaatg cagctgctgc tgatggccct ggcatttcac
     2881 agcttggcga agtggctcct ctccccaccc tgtctgctcc tgtgatggag cccctcatta
     2941 attctgagcc ttccactggt gtgactgagc cgctaacgcc tcatcagcca aaccaccagc
     3001 cctccttcct cacctctcct caagaccttg cctcttctcc aatggaagaa gatgagcagc
     3061 attctgaagc agatgagcct ccatcagacg aacccctatc tgatgacccc ctgtcacctg
     3121 ctgaggagaa attgccccac attgatgagt attggtcaga cagtgagcac atctttttgg
     3181 atgcaaatat tggtggggtg gccatcgcac ctgctcacgg ctcggttttg attgagtgtg
     3241 cccggcgaga gctgcacgct accactcctg ttgagcaccc caaccgtaat catccaaccc
     3301 gcctctccct tgtcttttac cagcacaaaa acctaaataa gccccaacat ggttttgaac
     3361 taaacaagat taagtttgag gctaaagaag ctaagaataa gaaaatgaag gcctcagagc
     3421 aaaaagacca ggcagctaat gaaggtccag aacagtcctc tgaagtaaat gaattgaacc
     3481 aaattccttc tcataaagca ttaacattaa cccatgacaa tgttgtcacc gtgtcccctt
     3541 atgctctcac acacgttgcg gggccctata accattgggt cggatccgcc accatgaaaa
     3601 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagaagatg gacaaaaaga
     3661 cgattgtgtg gtttagacgt gaccttcgca ttgaggacaa ccctgcgttg gcagcggcag
     3721 cgcatgaagg ttcggtattc cctgtcttta tctggtgtcc cgaagaggag gggcaattct
     3781 atcccggtcg cgcgtcccga tggtggatga aacagtcgct cgcacatctt agccagtcgt
     3841 tgaaggcgct cggctctgat ctgacgctca tcaaaacaca caatacaatt tctgccattc
     3901 tggattgcat tagggtgact ggtgccacca aagttgtgtt caatcacctt tatgatcccg
     3961 taagcctggt cagggatcat acagtaaaag agaaacttgt agagagggga atcagcgtcc
     4021 aatcgtacaa tggggacttg ttgtatgaac cgtgggaaat ctattgtgag aaaggcaaac
     4081 ctttcacttc cttcaattcg tactggaaga aatgcctcga catgtccatc gagtcagtca
     4141 tgctgccgcc accttggaga cttatgccca ttactgctgc ggcagaggcg atctgggctt
     4201 gcagcatcga agaacttggt ctggaaaacg aagcggaaaa acccagtaac gcgctcctga
     4261 cgcgagcgtg gtcgccaggc tggtcgaatg ctgataagct ccttaatgag tttatcgaga
     4321 aacagttgat cgactatgcg aagaatagca agaaggtggt gggaaattcc acttccctcc
     4381 tgtcgccgta tctccacttt ggggagatta gcgtgaggca tgtgttccaa tgtgcgcgga
     4441 tgaagcaaat catttgggct cgcgacaaaa actctgaagg agaggagtct gccgatttgt
     4501 ttttgagagg aatcggcctt agggagtatt cacgatacat ctgcttcaac tttccgttta
     4561 cacatgaaca atccctcctc agccacctcc gcttctttcc gtgggacgcg gacgtggaca
     4621 aattcaaggc ctggcggcag gggcgcacgg gatacccgtt ggtggatgcg ggtatgagag
     4681 agctgtgggc aacgggttgg atgcacaacc gtattcgagt catcgttagt agctttgccg
     4741 ttaagttcct cttgttgccc tggaaatggg ggatgaaata cttttgggac acgcttctgg
     4801 atgcggactt ggagtgcgat attcttgggt ggcagtacat ttcgggttcc atccctgacg
     4861 ggcatgaact tgaccgattg gataaccctg cactgcaagg agcaaagtac gacccggagg
     4921 gagaatacat caggcagtgg ctcccagagc tggcccgtct gcccaccgag tggattcacc
     4981 acccgtggga tgctcccctg accgtcctta aagcctcagg ggtcgaattg gggacgaatt
     5041 acgcgaaacc catcgtcgat attgacaccg cacgagaatt gctggcgaaa gccatctcaa
     5101 gaacacgcga agcgcagatt atgatcggag cagcgccggc tagccccaag aagaagagaa
     5161 aggtggaggc cagcggttcc ggacgggctg acgcattgga cgattttgat ctggatatgc
     5221 tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg
     5281 actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctga
     5341 ttaactctag aggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     5401 agaatcctgg cccagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5461 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5521 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5581 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5641 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5701 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5761 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5821 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5881 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5941 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6001 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6061 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6121 tgtacaagta agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     6181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6541 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6721 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     6781 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     6841 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     6901 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     6961 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     7021 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     7081 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     7141 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     7201 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     7261 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     7321 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     7381 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     7441 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     7501 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7681 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     7741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7801 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     7861 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7921 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     7981 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8041 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8101 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8161 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     9541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10021 ctcacatgt
//
