LOCUS       sequence-156877-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian GFP reporter plasmid, target site for Vika recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Karimova M, Abi-Ghanem J, Berger N, Surendranath V, Pisabarro MT, 
            Buchholz F
  TITLE     Vika/vox, a novel efficient and specific Cre/loxP-like site-specific
            recombination system.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):e37. doi: 10.1093/nar/gks1037. 
            Epub 2012 Nov 9.
  PUBMED    23143104
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-01"; volume: "41(2):e37. doi"; pages: " 
            10.1093/nar/gks1037. Epub 2012 Nov 9"
COMMENT     Sequence Label: pRK5-vox-EGFP
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(71..428)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      85..220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        237..428
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     385..405
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(430..534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(661..680)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(693..711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(694..711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(921..945)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(942..993)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(971..991)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
       61 ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
      121 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      181 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      241 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      301 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      361 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
      421 cgcctcagga ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      481 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      541 tccccgaaaa gtgccaccta agcttaatac gtctgagaat gggcgttctc agacctatta
      601 gatctttcga agtcgaattc aatcgataga accgaggtgc agttggacct gggagtggac
      661 acctgtggag agaaaggcaa agtggatgtt attctatagt gtcacctaaa tcgtatgtgt
      721 atgatacata aggttatgta ttaattgtag ccgcgttcta acgaagccaa gggggtgggc
      781 ctatagactc tataggcggt acttacgtca ctcttggcac ggggaatccg cgttccaatg
      841 caccgttccc ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
      901 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc
      961 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt ac
//
