LOCUS       sequence-156893-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein splicing reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Orengo JP, Bundman D, Cooper TA
  TITLE     A bichromatic fluorescent reporter for cell-based screens of 
            alternative splicing.
  JOURNAL   Nucleic Acids Res. 2006;34(22):e148. Epub 2006 Nov 16.
  PUBMED    17142220
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(22)"; pages: "e148. Epub 2006
            Nov 16"
COMMENT     Sequence Label: RG6
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ccatggatta caaggatgac
      121 gatgacaagg gggtacctgc cccaaaaaaa aaacgcaaag tggaggaccc agtaccagga
      181 tctagaggta ggtgatcctc ctgctgcttt ggttcagggt tttgcttgag gggggggggt
      241 ggtgatttcc ttgccatggg cagactgagc agaaaaggcc attgggacca tgttctgaat
      301 gcctccacct caaccaccgg ccggtaggac caaagccacc ccgtgttttc tcaggatctc
      361 ttttcccagg gagatccctc ggcccaaaga gggagatggc aatgctggat gtgtgcacaa
      421 taattcaaca ggcattggaa cttcagcatc gatgctgaat gcaattaaca atgctcaagc
      481 agaacccccg gctccatcag cacagtgcag gaccaaaccc catgctgcag cagtggggct
      541 gtctgtacgg ggtgggcaat gggaaccggg gtctgctggg gctcctgctg cttcagtgct
      601 gccatgcagc cacacatcct gagagctgaa agggtcggcg tcctcacctg gtgcacaccg
      661 tagctctgcc ccacagcttt aaggcacctg gctaacctct gcgcttcttc ccttccctcc
      721 tccctggctc aggatccagg cgatatccgg aagaattcag gtagttactg cacctttctt
      781 tgttccatct ctccacctct gctgtgaata aatcgcgggt cggtgtgtcc tgtgcctttc
      841 c
//
