LOCUS       sequence-156916-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE78
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..148)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      121 aaagttggaa cctcttacgt gccgatcaac gtctcaggtc tctgcaagaa ttcaagcttg
      181 gagattacgg atatgaatat aggcatatcc gtatccgaat tatccgtttg acagctagca
      241 acgattgtac aattgcttct ttaaaaaagg aagaaagaaa gaaagaaaag aatcaacatc
      301 agcgttaaca aacggccccg ttacggccca aacggtcata tagagtaacg gcgttaagcg
      361 ttgaaagact cctatcgaaa tacgtaaccg caaacgtgtc atagtcagat cccctcttcc
      421 ttcaccgcct caaacacaaa aataatcttc tacagcctat atatacaacc cccccttcta
      481 tctctccttt ctcacaattc atcatctttc tttctctacc cccaatttta agaaatcctc
      541 tcttctcctc ttcattttca aggtaaatct ctctctctct ctctctctct gttattcctt
      601 gttttaatta ggtatgtatt attgctagtt tgttaatctg cttatcttat gtatgcctta
      661 tgtgaatatc tttatcttgt tcatctcatc cgtttagaag ctataaattt gttgatttga
      721 ctgtgtatct acacgtggtt atgtttatat ctaatcagat atgaatttct tcatattgtt
      781 gcgtttgtgt gtaccaatcc gaaatcgttg a
//
