LOCUS       sequence-156919-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DEST vector for cell-free in vitro expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     pAM-PAT-ProUBQ10 GW
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOLENTE
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(69..91)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     191..210
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(277..732)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(414..435)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     626..645
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(811..858)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
       61 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      121 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      181 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      241 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
      301 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      361 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
      421 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
      481 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
      541 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
      601 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
      661 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
      721 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      781 ggcctcttcg ctattacgcc agcccggatc caaaaaaccc ctcaagaccc gtttagaggc
      841 cccaaggggt tatgctag
//
