LOCUS       sequence-156923-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Entry vector containing a temperature-sensitive mouse DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS, 
            Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A, 
            Dissmeyer N
  TITLE     Phenotypes on demand via switchable target protein degradation in 
            multicellular organisms.
  JOURNAL   Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202.
  PUBMED    27447739
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-22"; volume: "7:12202. doi"; pages: " 
            10.1038/ncomms12202"
COMMENT     Sequence Label: pEN-L1-K2-L2
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             367..927
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMATTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEDKGIKYKFEVYEKKD"
     CDS             943..969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag caggcttact cgagctgcag aattactatt tacaattaca
      121 atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctagaggt tgagtcttca
      181 gacaccatcg acaacgttaa ggctaaaatc caggacaagg aaggcattcc acctgatcaa
      241 caaagattga tctttgccgg taagcagctc gaggacggta gaactcttgc tgactacaat
      301 attcagaagg agtcgacctt acatcttgtc ttaagactta gaggtgggtt ccacggatct
      361 ggaatcatgg ttcgaccatt gaactgcatc gtcgccgtgt cccaaaatat ggggattggc
      421 aagaacggag acctaccctg gcctccgctc aggaacgagt tcaagtactt ccaaagaatg
      481 gccacaacct cttcagtgga aggtaaacag aatctggtga ttatgggtag gaaaacctgg
      541 ttctccattc cggagaagaa tcgaccttta aaggacagaa ttaatatagt tctcagcagg
      601 gaactcaaag agccaccacg aggagctcat tttcttgcca aaagtttgga tgatgcctta
      661 agacttattg aacaaccgga attggcaagt aaagtagaca tggtttggat agtcggaggc
      721 agttctgttt accaggaagc catgaatcaa ccaggccacc tcagactctt tgtgacaagg
      781 atcatgcagg aatttgaaag tgacacgttt ttcccagaaa ttgatttggg gaaatataaa
      841 cttctcccag aatacccagg cgtcctctct gaggtccagg aggacaaagg catcaagtat
      901 aagtttgaag tctacgagaa gaaagacggt tctatgggat cctacccata cgatgttcct
      961 gactatgcgg gctat
//
