LOCUS       sequence-156924-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Entry vector containing a temperature-sensitive mouse DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS, 
            Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A, 
            Dissmeyer N
  TITLE     Phenotypes on demand via switchable target protein degradation in 
            multicellular organisms.
  JOURNAL   Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202.
  PUBMED    27447739
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-22"; volume: "7:12202. doi"; pages: " 
            10.1038/ncomms12202"
COMMENT     Sequence Label: pEN-L1-K2-L2
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(104..193)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(209..769)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMATTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEDKGIKYKFEVYEKKD"
ORIGIN
        1 tttattttga ctgatagtga cctgtttcgt tgcaacaaat tgataagcaa tgctttctta
       61 taatgccaac tttgtacaag aaagctgggt aagcaccagc accagcgtaa tctggaacgt
      121 cgtatggata ggatcctgca tagtccggga cgtcataggg atagcccgca tagtcaggaa
      181 catcgtatgg gtaggatccc atagaaccgt ctttcttctc gtagacttca aacttatact
      241 tgatgccttt gtcctcctgg acctcagaga ggacgcctgg gtattctggg agaagtttat
      301 atttccccaa atcaatttct gggaaaaacg tgtcactttc aaattcctgc atgatccttg
      361 tcacaaagag tctgaggtgg cctggttgat tcatggcttc ctggtaaaca gaactgcctc
      421 cgactatcca aaccatgtct actttacttg ccaattccgg ttgttcaata agtcttaagg
      481 catcatccaa acttttggca agaaaatgag ctcctcgtgg tggctctttg agttccctgc
      541 tgagaactat attaattctg tcctttaaag gtcgattctt ctccggaatg gagaaccagg
      601 ttttcctacc cataatcacc agattctgtt taccttccac tgaagaggtt gtggccattc
      661 tttggaagta cttgaactcg ttcctgagcg gaggccaggg taggtctccg ttcttgccaa
      721 tccccatatt ttgggacacg gcgacgatgc agttcaatgg tcgaaccatg attccagatc
      781 cgtggaaccc acctctaagt cttaagacaa gatgtaaggt cgactccttc tgaatattgt
      841 agtcagcaag agttctaccg tcctcgagct gcttaccggc aaagatcaat ctttgttgat
      901 caggtggaat gccttccttg tcctggattt tagccttaac gttgtcgatg gtgtctgaag
      961 actcaacctc tagagtta
//
