LOCUS       sequence-156926-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE78
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccttagtcag attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc
       61 aataattttt cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat
      121 ttttatcgga tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg
      181 gcacgatatg atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt
      241 acatgtccct gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt
      301 tttcaactgg gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc
      361 tcttcatcct ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca
      421 tctcgataac gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga
      481 ccttaacggt ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt
      541 gctcgtggag actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg
      601 tgagagagtc atcatggatc aactgcatga agttccggtt ggcaaatcca tcggacttaa
      661 gaaaatccag gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg
      721 acagccgccc ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga
      781 agagatgagc gtaagttttc aagcgttctt caatcatctc cctatcttca aacaacgtaa
      841 gggtgaggac aatgtcctca agaatgtcct cgttctcctc attgtccagg aagtccttgt
      901 ctttaatgat tttcaggaga tcgtgatacg tt
//
