LOCUS       sequence-156928-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE78
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgttattt acgtatgtat atatgtagat ctggactttt tggagttgtt gacttgattg
       61 tatttgtgtg tgtatatgtg tgttctgatc ttgatatgtt atgtatgtgc agcaatggct
      121 cctaagaaga agcggaaggt tggtggcatg gacaagaagt actccattgg gctcgctatc
      181 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa
      241 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc
      301 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga
      361 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct
      421 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa
      481 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag
      541 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg
      601 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag
      661 ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact
      721 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc
      781 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg
      841 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccc
//
