LOCUS       sequence-156942-        7803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Pesch M, Schultheiss I, Digiuni S, Uhrig JF, Hulskamp M
  TITLE     Mutual control of intracellular localisation of the patterning 
            proteins AtMYC1, GL1 and TRY/CPC in Arabidopsis.
  JOURNAL   Development. 2013 Aug;140(16):3456-67. doi: 10.1242/dev.094698.
  PUBMED    23900543
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-08"; volume: "140"; issue: 
            "16"; pages: "3456-67"
COMMENT     Sequence Label: pAM PAT-ProTRY GW
FEATURES             Location/Qualifiers
     source          1..7803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..26
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(120..372)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(392..943)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(985..1164)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    2788..2912
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2937..2942,2943..2960,2961..2967)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3021..3680
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3087..3106)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4022..4327
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4206..4225
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4368..4492)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4863..4887
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4999..5708
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5769..5873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5874..5942,5943..6734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6092..6111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6905..7493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7394..7413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
       61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
      121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
      421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
      481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
      541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
      601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
      661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
      721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
      781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
      841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
      901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
      961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
     1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
     1261 ccgccttcgg tttgggcgcg cctcttagct tcgaagaaaa cgcaaatcag ggattttggt
     1321 tgtatcatgt gtgagagatt gaatttctcc agaagcactc attaaatcgc gtgtgtaagg
     1381 agttgccata cagcgtcgta tgtaatgtct tagtcactgt tctagaatta aaacatatgt
     1441 caaatgtaga attaaaaaac aggggttaaa atgttaaatt attcggagaa gtggaattat
     1501 tatattaaaa acattgtgtg attttgggat aaaatctgaa tcatgttttt tattgtgcca
     1561 aaaaatttct gtttcaacct cttcgtggag atgaaagaaa agtcaatcgg gaatgagact
     1621 tcttgtataa cgaatgaaaa tatgaacatg ttgattaatg aattaattaa catacgtact
     1681 atattaagtg ggaatattag gtataggcct tcgattttcc gttttgtttg gttttttcga
     1741 ttttaataat atttctctaa attaaaataa attttagttt ggtttattta aaatattaat
     1801 aaaataatat aaataactat ctaattaata tattaaaaag tattttctat atatattgat
     1861 ataaatatta ttcccatgtt ttatattggg tgacgttatc ttttaagtaa agttgtaaga
     1921 tatctaactt attcctacat tataataact atcaaaataa aataactaat tgtagtgaca
     1981 atgatgacta tcaaaatatg tatgtgtttt ggctcaaaca tagcgtttaa taacaaacac
     2041 aatggacaat tttaataaat ttgatcgatt agattttgtt tcattttcat gcactagatt
     2101 aaggttcgaa ttagattcat tttagtgtat atagtgaaac taatttgtta ctatatggaa
     2161 aaaaaaataa gtttattttt ggttcaagaa tttttattcg gtacggtttg gtgtgtatgt
     2221 ccaaccctga aaaaaaaact tgaaaaacta ataacctaat aacaactaat tttcttagaa
     2281 atacaaattg gacttgaata caaaaatgga aagaacaaca acaaatcaat aactaaaaca
     2341 tcgttgaact tgcatctgcc aaataaaaat caataaatcc cattattcta aaaagatagt
     2401 aggtttagaa aaattgaata aatggaaaaa aaaaattgtt aggatctacg aatgagaaca
     2461 aagttagcct tcaaaatact tacaaatccc aataaaagac ttcatctcca tgtgtatttg
     2521 agtgtcaacg acaagtctac acaaagggta agaggtcaac aagaccacac aacacttctt
     2581 actattagtt ttgcaaaggc cgttcgttgg acatttcctt ctctctcctc ccctcttctt
     2641 cttcttgttc gctctataaa ctctcatctc tcacgtcttt ttttccttac attctccaaa
     2701 ctcaaaattt catcacatta atttctctct atttttcttt tcttacttca atagtcgacc
     2761 tcgagaaaga ggatccacct gaggatcaca agtttgtaca aaaaagctga acgagaaacg
     2821 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     2881 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     2941 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     3001 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     3061 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     3121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     3181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     3241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     3301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     3361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     3421 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     3481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     3541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     3601 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     3661 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg
     3721 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     3781 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     3841 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     3901 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     3961 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga
     4021 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     4081 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     4141 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     4201 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     4261 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     4321 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     4381 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     4441 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatcctcc
     4501 gttccatggg ctagaagctt gttgagaatt catcgcccgg gactgggtta acttccgatc
     4561 gattctagac tagttctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc
     4621 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt
     4681 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     4741 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga ccgggcggcc
     4801 gccaccgcgg tggaggggga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     4861 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     4921 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgt acatcaccga
     4981 cgagcaaggc aagaccgagc gcctttccga cgctcaccgg gctggttgcc ctcgccgctg
     5041 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
     5101 gacaccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     5161 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     5221 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     5281 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     5341 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     5401 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     5461 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     5521 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     5581 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     5641 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     5701 aatggcagcg ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat
     5761 cagcccgaca agctacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5821 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5881 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5941 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6001 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6061 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6121 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6181 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6241 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6301 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6361 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6421 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6481 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6541 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6601 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6661 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6721 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6781 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6841 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6901 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6961 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7021 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7441 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7501 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggactcta
     7561 gctagaggat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     7621 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     7681 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     7741 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     7801 ctg
//
