LOCUS       sequence-156944-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LOV2 anchored to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-NTOM20-LOV2
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..109
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(199..222)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(229..261)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(301..729)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     promoter        complement(855..964)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg
       61 ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat cgatctcagt
      121 ggtatttgtg agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca
      181 cctgaggtta atcacttagt gatggtgatg gtgatggtgg tgctcgagat cctcggggtc
      241 ttccggggcg agttctggct ggcttgcacg tgtatacagc tgtgcggccg caagctttta
      301 aagttctttt gccgcctcat caatattttc tgcagttttc ttaatcagca tgactccctc
      361 tctctcggca gcatctcgga catgctcagt tccatccaac tgaaccccaa taaagtactg
      421 gacatctccc ttctgatctc gcataggctg caagtgaaag aggttccaga actttttacc
      481 actctttgta taattaatca gctgaacagt gacctctgtt tggttatcta tggcatctct
      541 aatttttctc actgtcgcgc gatcagtttc aggaccttgt agaaacctgc agtttcttcc
      601 caaaatttct tcacggctat attctgtcaa ctgcaagaaa ctatcggacg cgaatataat
      661 gggattatct ggcaatcttg ggtcagtaat gacaaagttc ttctcaatac gttcaagtgt
      721 agtagccaat ctagatttaa agttcggatc gctgcggcgt ttgcgatcaa agtaaatgca
      781 atagccaatg aacagcgcgc cgcacactcc agctgcaatc gcgctgttgc ggcccaccat
      841 ggtatatctc ctttgattgt aaataaaatg taatttacag tatagtattt taattaatat
      901 acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa
      961 ggtcccggat taactccggg
//
