LOCUS       sequence-156948-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Zdk1 anchored to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-NTOM20-mVenus-Zdk1
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(200..223)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(230..262)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(302..325)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(332..352)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(359..376)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(395..571)
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
     CDS             complement(590..841)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(632..653)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     981..1000
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat
       61 gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga tcgatctcag
      121 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
      181 acctgaggtt aatcacttag tgatggtgat ggtgatggtg gtgctcgaga tcctcggggt
      241 cttccggggc gagttctggc tggcttgcac gtgtatacag ctgtgcggcc gcaagctttt
      301 atttgtcgtc gtcgtccttg tagtcgcggc cgccctggaa atacagattt tcaccgccat
      361 gatgatgatg atgatggcta ccaccagaac caccttttgg ggcctgggca tcgttcagtt
      421 ttttggcttc ggccagcaga ttggcgctct gagatggatc atcaaacagg ctcacgatga
      481 aggcaaactt ctgctcaaca ttcaggtttg gcagagaatg gatttccgca ccggcacgcg
      541 tcttttcttt attgaatttg ttatccacca tggatccact tccagaaccc ttgtacagct
      601 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      661 gcttctcgtt ggggtctttg ctcagtttgg actggtagct caggtagtgg ttgtcgggca
      721 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      781 cgccgtcctc gatgttgtgg cggatcttga agtttgcctt gatgccgttc ttctgcttgt
      841 cggcggtgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      901 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      961 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     1021 cctggacgta gccttcgggc atgg
//
